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阴道根治性子宫颈切除术术后妊娠的微生物学研究：采用 FFPE 胎盘标本 16S rRNA 测序。

Microbiological investigation of pregnancies following vaginal radical trachelectomy using 16S rRNA sequencing of FFPE placental specimens.

机构信息

Department of Obstetrics and Gynecology, Sapporo Medical University School of Medicine, Sapporo, Japan.

Varinos Inc., Tokyo, Japan.

出版信息

FEBS Open Bio. 2024 Nov;14(11):1825-1836. doi: 10.1002/2211-5463.13892. Epub 2024 Sep 8.

DOI:10.1002/2211-5463.13892
PMID:39245884
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11532974/
Abstract

This study examined the risk of intrauterine infection associated with radical trachelectomy (RT) in early-stage cervical cancer patients. This procedure preserves fertility but is linked to increased risk of intrauterine infection due to cervical defects during pregnancy. DNA was extracted from the formalin-fixed paraffin-embedded (FFPE) placental specimens of 23 pregnant post-RT patients and 16S rRNA gene sequencing was used for bacterial identification. The prevalence of Lactobacillus crispatus and Burkholderia stabilis was significantly higher in the non-chorioamnionitis group. In contrast, alpha diversity analysis using the PD index showed significantly higher diversity in the chorioamnionitis group (P = 0.04). The demonstrated relationship between chorioamnionitis and microbial diversity affirms the importance of controlling the genital bacterial flora in pregnancies following RT.

摘要

本研究探讨了早期宫颈癌患者行根治性子宫颈切除术（RT）后发生宫内感染的风险。该手术保留了生育能力，但由于妊娠期间宫颈缺陷，增加了宫内感染的风险。从 23 例 RT 后妊娠的孕妇 FFPE 胎盘标本中提取 DNA，并进行 16S rRNA 基因测序以鉴定细菌。非绒毛膜羊膜炎组中 L. crispatus 和 B. stabilis 的患病率明显更高。相比之下，应用 PD 指数进行的 alpha 多样性分析显示，绒毛膜羊膜炎组的多样性明显更高（P=0.04）。绒毛膜羊膜炎与微生物多样性之间的关系证明了控制 RT 后妊娠期间生殖道细菌菌群的重要性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fddf/11532974/a5f528ab9f0f/FEB4-14-1825-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fddf/11532974/ef08f6e2f4b2/FEB4-14-1825-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fddf/11532974/38214a50a618/FEB4-14-1825-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fddf/11532974/a045372ad359/FEB4-14-1825-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fddf/11532974/a5f528ab9f0f/FEB4-14-1825-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fddf/11532974/ef08f6e2f4b2/FEB4-14-1825-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fddf/11532974/38214a50a618/FEB4-14-1825-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fddf/11532974/a045372ad359/FEB4-14-1825-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fddf/11532974/a5f528ab9f0f/FEB4-14-1825-g003.jpg

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本文引用的文献

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EClinicalMedicine. 2023 Jun 26;61:102070. doi: 10.1016/j.eclinm.2023.102070. eCollection 2023 Jul.
2
Possible Therapeutic Mechanisms and Future Perspectives of Vaginal Microbiota Transplantation.阴道微生物群移植的可能治疗机制及未来展望
Microorganisms. 2023 May 29;11(6):1427. doi: 10.3390/microorganisms11061427.
3
Potential values of formalin-fixed paraffin-embedded tissues for intratumoral microbiome analysis in breast cancer.
福尔马林固定石蜡包埋组织在乳腺癌瘤内微生物组分析中的潜在价值。
Heliyon. 2023 May 19;9(6):e16267. doi: 10.1016/j.heliyon.2023.e16267. eCollection 2023 Jun.
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Review, role of lactoferrin in preventing preterm delivery.综述：乳铁蛋白在预防早产中的作用。
Biometals. 2023 Jun;36(3):521-530. doi: 10.1007/s10534-022-00471-9. Epub 2022 Dec 10.
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The effectiveness of transabdominal cerclage placement via laparoscopy or laparotomy: a systematic review and meta-analysis.经腹腔镜或剖腹手术行腹内宫颈环扎术的有效性：一项系统评价和荟萃分析。
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J Microbiol Methods. 2022 May;196:106476. doi: 10.1016/j.mimet.2022.106476. Epub 2022 Apr 28.
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