Neppert J, Marquard F, Mueller-Eckhardt C
Eur J Immunol. 1985 Jun;15(6):559-63. doi: 10.1002/eji.1830150606.
Evidence is presented that monoclonal antibodies (mAb) against some human major histocompatibility complex (MHC) gene products and human sera containing HLA antibodies strongly inhibit immune phagocytosis of anti-D-sensitized red blood cells by human monocytes. w6.32HL, a mAb against a monomorphic class I antigen of high cell surface density, revealed the strongest inhibition among mAb reactive with MHC class I products. mAb with preferential reactivity for monomorphic and polymorphic DR and DQ epitopes (L203, L227, IIIE3, Tü22, Genox 3.53 and IV12) were noninhibitory. Definite inhibition was also apparent with a mAb against DRw52/MT2 (I-LR2) and with an antibody to class II antigens of high cell surface density (2MC3). Human sera containing HLA antibodies showed strong inhibition of immune phagocytosis up to a dilution of 1/1000. This inhibition could not be abrogated by platelet absorption. This indicates that human sera inhibiting immune phagocytosis may comprise at least two types of antibodies: cytotoxic HLA-specific antibodies which may or may not be inhibitory, and inhibitory antibodies against monocytic antigens not necessarily cytotoxic. These latter antibodies may recognize HLA DR or another as yet undefined gene product within, or closely associated to, the human MHC.
有证据表明，针对某些人类主要组织相容性复合体（MHC）基因产物的单克隆抗体（mAb）以及含有HLA抗体的人血清可强烈抑制人单核细胞对抗D致敏红细胞的免疫吞噬作用。w6.32HL是一种针对细胞表面密度高的单态性I类抗原的单克隆抗体，在与MHC I类产物反应的单克隆抗体中显示出最强的抑制作用。对单态性和多态性DR和DQ表位（L203、L227、IIIE3、Tü22、Genox 3.53和IV12）具有优先反应性的单克隆抗体无抑制作用。针对DRw52/MT2（I-LR2）的单克隆抗体以及针对细胞表面密度高的II类抗原的抗体（2MC3）也有明显的抑制作用。含有HLA抗体的人血清在稀释至1/1000时仍表现出对免疫吞噬作用的强烈抑制。这种抑制作用不能通过血小板吸附消除。这表明抑制免疫吞噬作用的人血清可能至少包含两种类型的抗体：可能具有或不具有抑制作用的细胞毒性HLA特异性抗体，以及针对不一定具有细胞毒性的单核细胞抗原的抑制性抗体。后一种抗体可能识别HLA DR或人类MHC内或与其密切相关的另一种尚未明确的基因产物。
