Neppert J, Mueller-Eckhardt C
Tissue Antigens. 1985 Jul;26(1):51-9. doi: 10.1111/j.1399-0039.1985.tb00934.x.
Binding of monoclonal antibodies (MAB) to human MHC coded class I antigens, w6.32HL or 61D2, specifically leads to clustering of these antigens and their assembly at one polar site on a cell, whereby these class I antigens tow class II-antigens with them into the polar cap revealing preexisting or arising molecular complexes in the membrane of the living cell. These class II antigens included DR (seen by MAB 2MC3, L203, L227, I-LR2, 109d6, SFR-3) and DQ (seen by Genox 3.53 and IVD12). In analogous cocapping experiments employing MAB to various class II antigens, interdependent surface mobility (bidirectional cocapping) could be demonstrated between DRw52 (MT2, seen by I-LR2) and DQw3 (MB3, seen by IVD12) as well as between DRw53 (MT3, seen by 109d6) and DQw3. There was incomplete interdependence between DRw52 or DRw53, respectively, and other DR antigens (seen by L227). These latter antigens were independent from DQ (seen by Genox 3.53 or IVD12). Analyzing pairs of monoclonal antibodies with various specificities to determinants on class I or II molecules a frequent finding was unidirectional cocapping: here a first antibody cocapped epitopes seen by a second, but this antibody did not cocap epitopes seen by the first. We conclude that epitopes are irregularly distributed on MHC coded molecules or on complexes of these molecules.
单克隆抗体（MAB）与人MHC编码的I类抗原w6.32HL或61D2结合，会特异性导致这些抗原聚集并在细胞的一个极性位点组装，由此这些I类抗原将II类抗原一起拖入极性帽，揭示活细胞膜中预先存在或新出现的分子复合物。这些II类抗原包括DR（可被单克隆抗体2MC3、L203、L227、I-LR2、109d6、SFR-3识别）和DQ（可被Genox 3.53和IVD12识别）。在使用针对各种II类抗原的单克隆抗体进行的类似共帽实验中，可以证明DRw52（MT2，可被I-LR2识别）和DQw3（MB3，可被IVD12识别）之间以及DRw53（MT3，可被109d6识别）和DQw3之间存在相互依赖的表面移动性（双向共帽）。DRw52或DRw53分别与其他DR抗原（可被L227识别）之间存在不完全的相互依赖性。这些后者的抗原与DQ（可被Genox 3.53或IVD12识别）无关。分析对I类或II类分子上的决定簇具有不同特异性的单克隆抗体对时，经常发现单向共帽现象：即第一种抗体共帽了第二种抗体所识别的表位，但第二种抗体没有共帽第一种抗体所识别的表位。我们得出结论，表位在MHC编码的分子或这些分子的复合物上分布不规则。
