Salam Abdul, Kaushik Kush, Mukherjee Bodhidipra, Anjum Farhan, Sapkal Goraksha T, Sharma Shagun, Garg Richa, Nandi Chayan Kanti
School of Chemical Sciences, Indian Institute of Technology Mandi HP-175075 India
School of Biosciences and Bioengineering, Indian Institute of Technology Mandi HP-175075 India.
Chem Sci. 2024 Sep 5;15(38):15659-69. doi: 10.1039/d4sc04638b.
Zinc (Zn) based fluorescent metal complexes have gained increasing attention due to their non-toxicity and high brightness with marked fluorescence quantum yield (QY). However, they have rarely been employed in super-resolution microscopy (SRM) to study live cells and dynamics of lysosomes. Here, we present an NIR emissive highly photostable Zn-complex as a multifaceted fluorescent probe for the long-term dynamical distribution of lysosomes in various cancerous and non-cancerous cells in live condition and embryogenic evolution in (). Apart from the normal fission, fusion, and kiss & run, the motility and the exact location of lysosomes at each point were mapped precisely. A notable difference in the lysosomal motility in the peripheral region between cancerous and non-cancerous cells was distinctly observed. This is attributed to the difference in viscosity of the cytoplasmic environment. On the other hand, along with the super-resolved structure of the smallest size lysosome (∼77 nm) in live , the complete embryogenic evolution of lysosomes and lysosome-related organelles (LROs) was captured. We were able to capture the images of lysosomes and LROs at different stages of , starting from a single cell and extending to a fully matured adult animal.
基于锌（Zn）的荧光金属配合物因其无毒、高亮度以及显著的荧光量子产率（QY）而受到越来越多的关注。然而，它们很少被用于超分辨率显微镜（SRM）来研究活细胞和溶酶体的动态变化。在此，我们展示了一种近红外发射且高光稳定性的锌配合物，作为一种多面荧光探针，用于在活细胞状态下研究各种癌细胞和非癌细胞中溶酶体的长期动态分布以及（此处原文括号内容缺失，无法完整翻译）中的胚胎发育演变。除了正常的裂变、融合以及吻合并跑现象外，还精确绘制了溶酶体在每个时间点的运动情况及其确切位置。明显观察到癌细胞和非癌细胞周边区域溶酶体运动存在显著差异。这归因于细胞质环境粘度的不同。另一方面，除了在活细胞中解析出最小尺寸溶酶体（约77纳米）的超分辨结构外，还捕捉到了溶酶体和溶酶体相关细胞器（LROs）完整的胚胎发育演变过程。我们能够捕捉到从单细胞开始到完全成熟的成年动物等不同阶段的溶酶体和LROs图像。
