School of Basic Sciences, Indian Institute of Technology Mandi, Mandi 175075, H.P., India.
BioX Centre, Indian Institute of Technology Mandi, Mandi 175075, H.P., India.
J Phys Chem Lett. 2020 Jul 16;11(14):5741-5748. doi: 10.1021/acs.jpclett.0c01354. Epub 2020 Jul 6.
The gold nanocluster (GNC), because of its interesting photoluminescence properties and easy renal clearance from the body, has tremendous biomedical applications. Unfortunately, it has never been explored for super-resolution microscopy (SRM). Here, we present a protein-conjugated red emissive GNC for super-resolution radial fluctuation (SRRF) of the lysosome in HeLa cells. The diameter of the lysosome obtained in SRRF is ∼59 nm, which is very close to the original diameter of the smallest lysosome in HeLa cells. Conjugation of protein to GNC aided in the specific labeling of the lysosome. We hope that GNC not only will replace some of the common dyes used in SRM but due to its electron beam contrast could also be used as a multimodal probe for several other correlative bioimaging techniques.
金纳米团簇（GNC）由于其有趣的光致发光特性和易于从体内清除肾脏，因此在生物医学中有巨大的应用。不幸的是，它从未被探索用于超分辨率显微镜（SRM）。在这里，我们提出了一种用于 HeLa 细胞溶酶体超分辨率径向波动（SRRF）的蛋白质偶联红色发光 GNC。在 SRRF 中获得的溶酶体的直径约为 59nm，非常接近 HeLa 细胞中最小溶酶体的原始直径。蛋白质与 GNC 的偶联有助于溶酶体的特异性标记。我们希望 GNC 不仅可以替代一些在 SRM 中常用的染料，而且由于其电子束对比度，也可以用作其他几种相关生物成像技术的多模态探针。
