荷瘤大鼠体内铟 - 奥克辛标记的腹腔单核细胞的分布研究。
Distribution studies of 111In-oxine-labeled peritoneal mononuclear cells in tumor-bearing rats.
作者信息
Abreo K, Lieberman L M, Moorthy A V
出版信息
Int J Nucl Med Biol. 1985;12(1):53-5. doi: 10.1016/0047-0740(85)90013-0.
We studied the distribution of 111In-labeled peritoneal mononuclear cells (PMC) in Sprague-Dawley rats with carcinosarcoma (CS) tumor. We obtained PMC from normal rats and rats pretreated with BCG or irradiated CS cells as antigenic stimulant. PMC were labeled in-vitro with 111In-oxine and transferred by tail-vein injection to rats bearing CS tumor. Twenty-four, 48 and 72 h after PMC transfer, we measured the accumulation of these cells in the CS tumor as a percentage of dose radioactivity per gram of tumor using an external gamma-ray camera. PMC from normal and BCG treated donor rats accumulated 0.4% and 0.46% dose per gram of CS tumor respectively. PMC from donor rats given killed CS cells accumulated significantly greater concentrations of 111In (0.79% dose per gram of CS tumor, P less than 0.025). Thus killed CS cells were able to sensitize the PMC of normal rats. 111In-oxine-labeling is an elegant procedure to study the distribution of mononuclear cells in tumors.
我们研究了111铟标记的腹膜单核细胞（PMC）在患有癌肉瘤（CS）肿瘤的Sprague-Dawley大鼠体内的分布情况。我们从正常大鼠以及用卡介苗（BCG）预处理或经照射的CS细胞作为抗原刺激物处理过的大鼠中获取PMC。PMC在体外用111铟-奥克辛进行标记，然后通过尾静脉注射转移到患有CS肿瘤的大鼠体内。在PMC转移后24、48和72小时，我们使用外部伽马射线相机测量这些细胞在CS肿瘤中的积累情况，以每克肿瘤剂量放射性的百分比表示。来自正常和经卡介苗处理的供体大鼠的PMC分别在每克CS肿瘤中积累了0.4%和0.46%的剂量。来自给予灭活CS细胞的供体大鼠的PMC积累了显著更高浓度的111铟（每克CS肿瘤0.79%的剂量，P小于0.025）。因此，灭活的CS细胞能够使正常大鼠的PMC致敏。111铟-奥克辛标记是研究单核细胞在肿瘤中分布的一种精妙方法。
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