Jones W J, Donnelly M I, Wolfe R S
J Bacteriol. 1985 Jul;163(1):126-31. doi: 10.1128/jb.163.1.126-131.1985.
The roles of methanofuran and tetrahydromethanopterin as carriers of C1 moieties in the reduction of carbon dioxide to methane were studied in representatives of diverse groups of methanogens, confirming that these roles, first reported for Methanobacterium thermoautotrophicum, are common for methanogenesis in general. Extracts of the methanogens tested converted formyl-methanofuran and methyl-tetrahydromethanopterin to methane; the extractable cofactors derived from the same methanogens, with one exception, complemented a methanofuran- and tetrahydromethanopterin-deficient enzyme system from M. thermoautotrophicum. The amounts of extractable methanofuran and tetrahydromethanopterin were determined for each representative methanogen.
在不同类群的产甲烷菌中研究了甲呋喃和四氢甲蝶呤作为C1基团载体在二氧化碳还原为甲烷过程中的作用,证实了这些首次在嗜热自养甲烷杆菌中报道的作用在一般产甲烷过程中是常见的。所测试的产甲烷菌提取物将甲酰甲呋喃和甲基四氢甲蝶呤转化为甲烷;除了一个例外,从相同产甲烷菌中提取的辅因子补充了来自嗜热自养甲烷杆菌的缺乏甲呋喃和四氢甲蝶呤的酶系统。测定了每种代表性产甲烷菌中可提取的甲呋喃和四氢甲蝶呤的量。