College of Animal Science and Technology, Ningxia University, Yinchuan, China.
Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Parasitol Res. 2024 Sep 9;123(9):318. doi: 10.1007/s00436-024-08341-8.
Several miRNA-based studies on Theileria-transformed bovine cells have been conducted; however, the mechanism by which transformed cells exhibit uncontrolled proliferation is not yet fully understood. Therefore, it is necessary to screen more microRNAs that may play a role in the transformation process of host cells infected with Theileria annulata to better understand the transformation mechanisms of Theileria-infected cells. RNA sequencing was used to analyze miRNAs expression in the host bovine lymphocytes infected with T. annulata at different time points after buparvaquone (BW720) treatment and DMSO treatment (control groups). Differential miRNAs related to cell proliferation and apoptosis were identified through comparison with gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) databases, and a regulatory network of miRNA-mRNA was constructed. In total, 272 differentially expressed miRNAs were found at 36, 60 and 72 h. The miRNAs change of bta-miR-2285t, novel-miR-622, bta-miR-2478, and novel-miR-584 were significant. Analysis of 27 of these co-differential expressed miRNAs revealed that 15 miRNAs were down-regulated and 12 miRNAs were up-regulated. A further analysis of the changes in the expression of each of these 27 miRNAs in the three datasets suggested that bta-miR-2285t, bta-miR-345-5p, bta-miR-34a, bta-miR-150, and the novel-miR-1372 had significantly changed. Predicted target genes for these 27 miRNAs were analyzed by KEGG and the results demonstrated that EZR, RASSF, SOCS1 were mainly enriched in the signaling pathway microRNAs in cancer. MAPKAPK2, RELB, FLT3LG, and GADD45B were mainly enriched in the MAPK signaling pathway, and some genes were enriched in Axon guidance. This study has provided valuable information to further the understanding of the regulatory function of miRNAs in the host microenvironment and host-parasite interaction mechanisms.
已经有几项基于 microRNA 的研究针对感染泰勒虫的牛转化细胞展开，但导致转化细胞失控增殖的机制尚未完全阐明。因此，有必要筛选更多可能在感染泰勒虫的宿主细胞转化过程中发挥作用的 microRNA，以更好地理解感染泰勒虫的细胞的转化机制。使用 RNA 测序分析了经布帕伐醌（BW720）处理和 DMSO 处理（对照组）的感染泰勒虫的宿主牛淋巴细胞在不同时间点的 microRNA 表达。通过与基因本体论（GO）、京都基因与基因组百科全书（KEGG）数据库比较，鉴定与细胞增殖和凋亡相关的差异 microRNA，并构建了 microRNA-mRNA 调控网络。共发现 36、60 和 72 h 时 272 个差异表达 microRNA。bta-miR-2285t、novel-miR-622、bta-miR-2478 和 novel-miR-584 的 microRNA 变化显著。对这 27 个共差异表达 microRNA 中的 27 个进行分析显示，15 个 microRNA 下调，12 个 microRNA 上调。进一步分析这 27 个 microRNA 在三个数据集的表达变化表明，bta-miR-2285t、bta-miR-345-5p、bta-miR-34a、bta-miR-150 和 novel-miR-1372 有明显变化。对这 27 个 microRNA 的预测靶基因进行 KEGG 分析，结果表明 EZR、RASSF、SOCS1 主要富集于癌症中的信号通路 microRNA；MAPKAPK2、RELB、FLT3LG 和 GADD45B 主要富集于 MAPK 信号通路，部分基因富集于轴突导向。本研究为进一步理解宿主微环境中 microRNA 的调控功能以及宿主-寄生虫相互作用机制提供了有价值的信息。
