Zanetti M, Liu F T, Rogers J, Katz D H
J Immunol. 1985 Aug;135(2):1245-51.
We report on the detection of the same idiotype (Id62) on the heavy and light chains of a mouse monoclonal autoantibody to thyroglobulin. This result was obtained by a simple, sensitive, and reproducible technique, namely immunoblot on nitrocellulose paper of electrophoretically separated heavy and light chains by using a panel of 125I-labeled anti-idiotype probes. These included polyclonal heterologous and syngeneic affinity-purified antibodies and a syngeneic monoclonal antibody. The immunoblot findings were confirmed through the solid-phase radioimmunoassay binding of the anti-idiotype probes to isolated H and L polypeptide chains prepared by conventional chromatography methods. Because anti-idiotype probes define a putative regulatory idiotype of the BALB/c autoimmune response to thyroglobulin, it is suggested that the simultaneous presence of the same idiotype on H and L chains may confer to antibody regulatory properties within the idiotypic network. The significance of the existence of the same idiotype on H and L chains and the implication of this finding for the regulation of the immune response to self antigens are discussed.
我们报告了在一种抗甲状腺球蛋白的小鼠单克隆自身抗体的重链和轻链上检测到相同独特型（Id62）的情况。这一结果是通过一种简单、灵敏且可重复的技术获得的，即使用一组125I标记的抗独特型探针，对经电泳分离的重链和轻链在硝酸纤维素纸上进行免疫印迹。这些探针包括多克隆异源和同源亲和纯化抗体以及一种同源单克隆抗体。通过抗独特型探针与通过传统色谱方法制备的分离的重链和轻链多肽链的固相放射免疫测定结合，证实了免疫印迹结果。由于抗独特型探针定义了BALB/c对甲状腺球蛋白自身免疫反应的一种假定调节独特型，因此有人提出，重链和轻链上同时存在相同独特型可能赋予抗体在独特型网络内的调节特性。本文讨论了重链和轻链上存在相同独特型的意义以及这一发现对自身抗原免疫反应调节的影响。
