采用放射免疫分析法测定血清和脑脊液中的补体成分C1q、C4和C3。

The determination of the complement components C1q, C4 and C3 in serum and cerebrospinal fluid by radioimmunoassay.

作者信息

Dujardin B C, Driedijk P C, Roijers A F, Out T A

出版信息

J Immunol Methods. 1985 Jun 25;80(2):227-37. doi: 10.1016/0022-1759(85)90024-9.

Abstract

Non-competitive 2-site radioimmunoassays (RIA) for the determination of the complement proteins C1q, C4 and C3 in cerebrospinal fluid (CSF) are described. The quantitative results of the RIAs were the same as those obtained by other assay methods: radial immunodiffusion and turbidimetry and, in the case of C4, the haemolytic assay. The concentrations of the complement proteins in paired CSF and serum samples from a group of 60 patients were measured, as well as those of albumin and IgG. The ratios (concentration in CSF)/(concentration in serum) of the complement proteins correlated poorly with that of albumin. In contrast, the ratio of IgG was significantly correlated with that of albumin. The ratios of the complement proteins were higher than might be expected on the basis of their molecular masses. This suggests that these proteins may be synthesized within the normal central nervous system.

摘要

本文描述了用于测定脑脊液（CSF）中补体蛋白C1q、C4和C3的非竞争性双位点放射免疫分析（RIA）。RIA的定量结果与通过其他分析方法获得的结果相同：放射免疫扩散法和比浊法，对于C4而言，还有溶血分析法。测量了一组60名患者配对的脑脊液和血清样本中补体蛋白的浓度，以及白蛋白和IgG的浓度。补体蛋白的（脑脊液中浓度）/（血清中浓度）比值与白蛋白的该比值相关性较差。相比之下，IgG的比值与白蛋白的比值显著相关。补体蛋白的比值高于基于其分子量所预期的比值。这表明这些蛋白可能在正常中枢神经系统内合成。