Morhenn V B, Nickoloff B J, Mansbridge J N
J Invest Dermatol. 1985 Jul;85(1 Suppl):27s-29s. doi: 10.1111/1523-1747.ep12275425.
Recombinant human gamma interferon (r-IFN-gamma) induces the synthesis and expression of HLA-DR antigen on cultured, normal, human keratinocytes depleted of Langerhans cells. After removal of r-IFN-gamma from the culture medium of keratinocytes that are expressing HLA-DR antigen, the cells continue to express this antigen for at least 2 days. r-IFN-gamma induces, in a dose dependent fashion, the synthesis of several triton-soluble proteins with the most prominent having an apparent molecular weight of 53,000. Whereas normal keratinocytes do not express HLA-DR antigen in vivo, they do express HLA-DR in a variety of skin diseases such as lichen planus, graft-versus-host disease, and mycosis fungoides. We propose that an understanding of lymphocyte-keratinocyte interactions in vivo may be achieved by further studies of the mechanism of action of r-IFN-gamma on cultured keratinocytes and that the results may provide insight into the patho-physiology leading to a number of common inflammatory and neoplastic skin diseases.
重组人γ干扰素（r-IFN-γ）可诱导培养的、去除朗格汉斯细胞的正常人角质形成细胞合成并表达HLA-DR抗原。从表达HLA-DR抗原的角质形成细胞培养基中去除r-IFN-γ后，细胞继续表达该抗原至少2天。r-IFN-γ以剂量依赖方式诱导几种可被曲拉通溶解的蛋白质合成，其中最显著的一种蛋白质表观分子量为53,000。正常角质形成细胞在体内不表达HLA-DR抗原，但在多种皮肤疾病如扁平苔藓、移植物抗宿主病和蕈样肉芽肿中会表达HLA-DR。我们认为，通过进一步研究r-IFN-γ对培养角质形成细胞的作用机制，可能实现对体内淋巴细胞与角质形成细胞相互作用的理解，且研究结果可能为导致多种常见炎症性和肿瘤性皮肤病的病理生理学提供见解。
