Basham T Y, Nickoloff B J, Merigan T C, Morhenn V B
J Interferon Res. 1985 Winter;5(1):23-32. doi: 10.1089/jir.1985.5.23.
Recombinant gamma interferon induces class II antigen (HLA-DR) biosynthesis and expression on normal cultured human keratinocytes. HLA-DR expression was not induced on keratinocytes by recombinant alpha or beta interferons in a similar dose range nor by Con A or PHA. HLA-DR (L243) expression, as determined by FACS analysis, was detected as early as 1-2 days after addition of r-IFN-gamma to the cultures and was maximal after 4-8 days. Keratinocytes were analyzed for expression of another class II antigen, HLA-DC (Leu-10). Little or no expression of Leu-10 (DC) was detectable on these cells although Fc receptors for the IgG1 isotype were increased. These data indicate a unique role for gamma interferon in the differential regulation of keratinocyte class II antigen biosynthesis and expression. Induction of HLA-DR on keratinocytes may be functionally important in expanding the number of antigen presenting cells in the skin for the induction of an immune response and/or targeting these keratinocytes for cytolysis.
重组γ干扰素可诱导II类抗原（HLA - DR）的生物合成，并使其在正常培养的人角质形成细胞上表达。在相似剂量范围内，重组α或β干扰素、刀豆蛋白A（Con A）或植物血凝素（PHA）均不能诱导角质形成细胞表达HLA - DR。通过荧光激活细胞分选术（FACS）分析确定，早在向培养物中添加重组γ干扰素后1 - 2天即可检测到HLA - DR（L243）的表达，4 - 8天后表达量达到最大。分析角质形成细胞中另一种II类抗原HLA - DC（Leu - 10）的表达情况。尽管IgG1同种型的Fc受体增加，但在这些细胞上几乎检测不到Leu - 10（DC）的表达。这些数据表明γ干扰素在角质形成细胞II类抗原生物合成和表达的差异调节中具有独特作用。角质形成细胞上HLA - DR的诱导在扩大皮肤中抗原呈递细胞数量以诱导免疫反应和/或将这些角质形成细胞靶向细胞溶解方面可能具有重要功能。
