用于检测尿道炎男性中淋病奈瑟菌非洲和亚洲菌株的DNA杂交方法的评估。

Evaluation of a DNA-hybridization method for detection of African and Asian strains of Neisseria gonorrhoeae in men with urethritis.

作者信息

Perine P L, Totten P A, Holmes K K, Sng E H, Ratnam A V, Widy-Wersky R, Nsanze H, Habte-Gabr E, Westbrook W G

出版信息

J Infect Dis. 1985 Jul;152(1):59-63. doi: 10.1093/infdis/152.1.59.

DOI:10.1093/infdis/152.1.59

Abstract

The 2.6-megadalton (MDa) cryptic plasmid and the 4.4-MDa beta-lactamase plasmid of Neisseria gonorrhoeae were radiolabeled with [32P] nucleotides and used as probes for direct detection of gonococci and beta-lactamase plasmids in urethral exudates from men with urethritis. The sensitivity and specificity of the DNA probes were compared with culture isolation of N. gonorrhoeae and biochemical tests of gonococcal isolates for beta-lactamase production. Of 216 urethral specimens, 180 were positive for N. gonorrhoeae by DNA probe and culture, 27 were negative by both tests, and 9 gave discordant results. Compared with culture and with the chromogenic cephalosporin assay, the sensitivity and the specificity of the DNA probe was 99% and 93% and that of the beta-lactamase probe assay was 91% and 96%, respectively. Electrophoresis of plasmids isolated from 90 gonococcal cultures showed that all contained the 2.6-MDa plasmid, 29 possessed a 3.2-MDa plasmid, 18 a 4.4-MDa beta-lactamase plasmid, and 11 had a 24.5-MDa conjugal plasmid. We conclude that the sensitivity of our DNA probes was comparable to that of culture for diagnosis of gonorrhea and to conventional tests for detection of beta-lactamase.

摘要

用[32P]核苷酸对淋病奈瑟菌的2.6兆道尔顿（MDa）隐蔽质粒和4.4兆道尔顿β-内酰胺酶质粒进行放射性标记，并将其用作探针，直接检测尿道炎男性尿道分泌物中的淋球菌和β-内酰胺酶质粒。将DNA探针的敏感性和特异性与淋病奈瑟菌的培养分离以及淋病奈瑟菌分离株β-内酰胺酶产生的生化试验进行比较。在216份尿道标本中，180份通过DNA探针和培养检测淋病奈瑟菌呈阳性，27份两项检测均为阴性，9份结果不一致。与培养和显色头孢菌素测定相比，DNA探针的敏感性和特异性分别为99%和93%，β-内酰胺酶探针测定的敏感性和特异性分别为91%和96%。对从90份淋病奈瑟菌培养物中分离的质粒进行电泳分析，结果显示所有培养物均含有2.6-MDa质粒，29份含有3.2-MDa质粒，18份含有4.4-MDaβ-内酰胺酶质粒，11份含有24.5-MDa接合质粒。我们得出结论，我们的DNA探针在淋病诊断中的敏感性与培养相当，在检测β-内酰胺酶方面与传统试验相当。