Tenover F C
Laboratory Service, Veterans Administration Medical Center, Seattle, Washington 98108.
Clin Microbiol Rev. 1988 Jan;1(1):82-101. doi: 10.1128/CMR.1.1.82.
Virtually all microorganisms contain some unique nucleotide sequences which can be the target of deoxyribonucleic acid probes. Probes have been used successfully to identify a wide variety of pathogens from the simple ribonucleic acid-containing polioviruses to the complex filarial worms Brugia malayi. Probe technology offers the clinical laboratory the potential both to extend the types of pathogens that can be readily identified and to reduce significantly the time associated with the identification of fastidious microorganisms. Over a dozen commercially prepared deoxyribonucleic acid probe tests are now available. This article explores the development of deoxyribonucleic acid probe tests and reviews the sensitivity, specificity, and predictive values of many of the diagnostic probes developed during the last several years. Prospects for newer, more sensitive detection systems for the products of hybridization reactions are also reviewed.
几乎所有微生物都含有一些独特的核苷酸序列,这些序列可以成为脱氧核糖核酸探针的靶标。探针已成功用于识别从简单的含核糖核酸的脊髓灰质炎病毒到复杂的丝状蠕虫马来布鲁线虫等各种各样的病原体。探针技术为临床实验室提供了潜力,既能扩展可以轻易识别的病原体类型,又能显著减少鉴定苛求微生物所需的时间。现在有十几种商业制备的脱氧核糖核酸探针检测方法可供使用。本文探讨了脱氧核糖核酸探针检测方法的发展,并回顾了过去几年开发的许多诊断探针的敏感性、特异性和预测价值。还综述了杂交反应产物更新、更灵敏检测系统的前景。