Laboratory of Environmental Biotechnology, Graduate School of Agricultural Science, Tohoku University, Sendai, 980-8572, Japan.
Laboratory of Integrated Genomic Breeding, Institute of Plant Science and Resources, Okayama University, Kurashiki, 710-0046, Japan.
Plant J. 2024 Nov;120(3):941-949. doi: 10.1111/tpj.17028. Epub 2024 Sep 9.
Cytoplasmic male sterility (CMS) is an agronomically significant trait that causes dysfunction in pollen and anther development. It is often observed during successive backcrossing between distantly related species. Here, we show that Asian japonica cultivars (Oryza sativa) exhibit CMS when the nucleus is replaced with that of the African rice Oryza glaberrima. The CMS line produced stunted anthers and did not set any seeds. Mitochondrial orf288 RNA was detected in the anthers of CMS lines but not in fertility restorer lines. The mitochondrial genome-edited japonica rice that was depleted of orf288 did not exhibit male sterility when backcrossed with O. glaberrima. These results demonstrate that orf288 is a CMS-causing gene. As orf288 commonly occurs in the mitochondrial genomes of japonica rice, these results indicate that common japonica rice cultivars possess a cryptic CMS-causing gene hidden in their mitochondrial genomes.
细胞质雄性不育（CMS）是一种在花粉和花药发育中导致功能障碍的重要农艺性状，通常在远缘物种的连续回交中观察到。在这里，我们表明，当亚洲粳稻品种（Oryza sativa）的核被非洲稻 Oryza glaberrima 的核取代时，会表现出 CMS。CMS 系产生的花药矮小，没有结任何种子。在 CMS 系的花药中检测到线粒体 orf288 RNA，但在育性恢复系中没有检测到。回交非洲稻 O. glaberrima 的线粒体基因组编辑粳稻耗尽 orf288 后，没有表现出雄性不育。这些结果表明 orf288 是 CMS 引起的基因。由于 orf288 通常存在于粳稻的线粒体基因组中，这些结果表明常见的粳稻品种在其线粒体基因组中隐藏着一个隐性 CMS 引起的基因。
