异质 stock 大鼠种群中的 Y 和线粒体染色体。

Y and mitochondrial chromosomes in the heterogeneous stock rat population.

机构信息

Department of Psychiatry, University of California San Diego, La Jolla, CA 92093, USA.

Bioinformatics and System Biology Program, University of California San Diego, La Jolla, CA 92093, USA.

出版信息

G3 (Bethesda). 2024 Nov 6;14(11). doi: 10.1093/g3journal/jkae213.

DOI:10.1093/g3journal/jkae213

PMID:39250761

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11540319/

Abstract

Genome-wide association studies typically evaluate the autosomes and sometimes the X Chromosome, but seldom consider the Y or mitochondrial (MT) Chromosomes. We genotyped the Y and MT Chromosomes in heterogeneous stock (HS) rats (Rattus norvegicus), an outbred population created from 8 inbred strains. We identified 8 distinct Y and 4 distinct MT Chromosomes among the 8 founders. However, only 2 types of each nonrecombinant chromosome were observed in our modern HS rat population (generations 81-97). Despite the relatively large sample size, there were virtually no significant associations for behavioral, physiological, metabolome, or microbiome traits after correcting for multiple comparisons. However, both Y and MT Chromosomes were strongly associated with the expression of a few genes located on those chromosomes, which provided a positive control. Our results suggest that within modern HS rats there are no Y and MT Chromosomes differences that strongly influence behavioral or physiological traits. These results do not address other ancestral Y and MT Chromosomes that do not appear in modern HS rats, nor do they address effects that may exist in other rat populations, or in other species.

摘要

全基因组关联研究通常评估常染色体，有时还评估 X 染色体，但很少考虑 Y 染色体或线粒体（MT）染色体。我们对异质品系（HS）大鼠（Rattus norvegicus）的 Y 和 MT 染色体进行了基因分型，该品系是由 8 个近交系创建的。在 8 个创始人中，我们发现了 8 种不同的 Y 染色体和 4 种不同的 MT 染色体。然而，在我们现代的 HS 大鼠群体（第 81-97 代）中，只观察到每种非重组染色体的 2 种类型。尽管样本量相对较大，但在进行多次比较校正后，行为、生理、代谢组或微生物组特征几乎没有显著的关联。然而，Y 染色体和 MT 染色体都与位于这些染色体上的少数基因的表达强烈相关，这提供了一个阳性对照。我们的结果表明，在现代 HS 大鼠中，没有 Y 染色体和 MT 染色体差异强烈影响行为或生理特征。这些结果并没有解决其他在现代 HS 大鼠中不存在的祖先 Y 染色体和 MT 染色体的问题，也没有解决可能存在于其他大鼠群体或其他物种中的影响问题。