Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education of China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, People's Republic of China.
Mikrochim Acta. 2024 Sep 9;191(10):585. doi: 10.1007/s00604-024-06671-z.
A new method of reducing the amount of reagent and sample for determination of thrombomodulin (TM) was developed based on competitive immunoreaction using a portable glucometer (PGM). Two types of nanocomposites, TM protein-modified magnetic nanoparticles (MNPs-TM) and TM antibody-/glucose oxidase-modified gold nanoparticles (Ab-GNPs-GOx), were prepared. Their binding product, MNPs-TM-Ab-GNPs-GOx, in the microvolumetric solution was used to catalyze the oxidation of glucose, leading to a decline of the glucose content. The TM-involved competitive immunoreaction had a negative effect on the generation of MNPs-/GNPs-based nanocomposites and inhibited the catalytic oxidation of glucose. The glucose content difference in the microvolumetric solution, which was revealed by a PGM, was in proportion to the logarithm of the TM concentration from 25 ng mL to 2.5 μg mL. The limit of detection was 5.7 ng mL. Microvolumetric solution and a PGM were used in the measurement, which overcame some deficiencies of classical methods in chemo/biosensing, for example, special instrument, complicated measurement procedure, and high cost.
基于使用便携式血糖仪（PGM）的竞争免疫反应，开发了一种新的方法来减少血栓调节蛋白（TM）测定所需的试剂和样品量。制备了两种类型的纳米复合材料，即 TM 蛋白修饰的磁性纳米颗粒（MNPs-TM）和 TM 抗体/葡萄糖氧化酶修饰的金纳米颗粒（Ab-GNPs-GOx）。在微体积溶液中，它们的结合产物 MNPs-TM-Ab-GNPs-GOx 用于催化葡萄糖的氧化，导致葡萄糖含量下降。TM 参与的竞争免疫反应对 MNPs-/GNPs 基纳米复合材料的生成有负面影响，并抑制了葡萄糖的催化氧化。通过 PGM 揭示的微体积溶液中的葡萄糖含量差异与 25ngmL 至 2.5μgmL 的 TM 浓度的对数成正比。检测限为 5.7ngmL。微体积溶液和 PGM 用于测量，克服了化学/生物传感中经典方法的一些缺陷，例如特殊仪器、复杂的测量程序和高成本。
