Department of Surgical Sciences, University of Wisconsin-Madison, School of Veterinary Medicine, Madison, WI 53706, USA,
Department of Surgical Sciences, University of Wisconsin-Madison, School of Veterinary Medicine, Madison, WI 53706, USA.
J Zoo Wildl Med. 2024 Sep;55(3):565-572. doi: 10.1638/2023-0101.
Acute phase proteins (APP) and protein electrophoresis (EPH) offer crucial insights into inflammation and overall health in various species. In this study, we validated serum amyloid A (SAA) and C-reactive protein (CRP) reagents for use with serum samples from gibbons (Hylobatidae, = 50), spanning five species across four gibbon genera: eastern hoolock (), Javan (), pileated (), siamang (), and white-cheeked (). Preliminary reference intervals ( = 50) were calculated for SAA (1.8-48.1 mg/L), CRP (0.1-11.1 mg/L), and EPH via capillary zone electrophoresis, in healthy gibbons. Comparing clinically normal ( = 38) and abnormal ( = 12) individuals, significant differences were observed in the albumin/globulin ratio ( = 0.0003), prealbumin ( = 0.0345), and albumin ( = 0.0094), with abnormal individuals exhibiting statistically significantly higher γ-globulins ( = 0.0224), SAA ( = 0.0001), and CRP ( = 0.0003). Despite significant chromosomal rearrangements among different gibbon species, we found no statistically significant differences of SAA and CRP levels across species. However, some differences between species were observed in EPH fractions. This study presents the first report of the evaluation of APP and EPH in gibbons, underscoring the potential use of these biomarkers in gibbon health monitoring. Further research with larger sample sizes of both normal and abnormal gibbons is recommended to solidify the clinical utility of these biomarkers in these species.
急性相蛋白（APP）和蛋白电泳（EPH）为各种物种的炎症和整体健康状况提供了重要的见解。在这项研究中，我们验证了血清淀粉样蛋白 A（SAA）和 C 反应蛋白（CRP）试剂在 50 只合趾猿（长臂猿科）血清样本中的应用，涵盖了四个合趾猿属的五个物种：东部合趾猿（）、爪哇合趾猿（）、白掌长臂猿（）、苏门答腊合趾猿（）和白颊长臂猿（）。通过毛细管区带电泳，在健康的合趾猿中计算了 SAA（1.8-48.1mg/L）、CRP（0.1-11.1mg/L）和 EPH 的初步参考区间。比较临床正常（n=38）和异常（n=12）个体，发现白蛋白/球蛋白比值（=0.0003）、前白蛋白（=0.0345）和白蛋白（=0.0094）存在显著差异，异常个体的γ-球蛋白（=0.0224）、SAA（=0.0001）和 CRP（=0.0003）显著升高。尽管不同合趾猿物种之间存在显著的染色体重排，但我们没有发现 SAA 和 CRP 水平在物种间存在统计学上的显著差异。然而，在 EPH 分数中观察到了一些物种间的差异。本研究首次报道了 APP 和 EPH 在合趾猿中的评估，强调了这些生物标志物在合趾猿健康监测中的潜在应用。建议对正常和异常合趾猿进行更大样本量的研究，以确定这些生物标志物在这些物种中的临床应用价值。
