Department of Crop Protection, Federal University of Pelotas (UFPel), Capão do Leão, Brazil.
Departament of Entomology, Bayer Crop Science Brazil, São Paulo, Brazil.
J Econ Entomol. 2024 Oct 14;117(5):2135-2142. doi: 10.1093/jee/toae200.
Rachiplusia nu Guenée is a polyphagous species able to develop on several cultivated and non-cultivated host plants. However, basic life history information about this pest on hosts is scarce. In this study, R. nu larvae did not survive on leaves of non-Bt corn, wheat, Bt cotton that expresses proteins Cry1Ac+Cry2Ab2 or on Intacta2 Xtend soybean that expresses the Cry1A.105/Cry2Ab2/Cry1Ac proteins. Rachiplusia nu showed a viable egg-to-adult biological cycle (54%-66.3%) on non-Bt soybean, sunflower, canola, vetch, Persian clover, alfalfa, bean, and forage turnip hosts, similar to larvae raised on the artificial diet. In addition, R. nu was unable to complete larval development on non-Bt cotton, and only 45.2% of R. nu larvae reached the pupal stage when fed leaves of intacta RR2 PRO soybean that expresses the Cry1Ac protein. Larval and pupal mass of surviving insects on Cry1Ac soybean leaves were also lower (larval: 0.104 g; pupal: 0.099 g) in relation to other food sources (larval: 0.165-0.189 g; pupal: 0.173-0.192 g). The total fecundity of R. nu on Cry1Ac soybean leaves was ≈65% lower in relation to other food sources. This fact caused ≈60% the net reproductive rate (Ro) and intrinsic rate of increase (rm) when compared to other food sources. Our findings indicate that the Cry1Ac soybean negatively affects the biological parameters of R. nu. Non-Bt soybean, sunflower, canola, vetch, Persian clover, alfalfa, bean, and forage turnip are viable food sources for the survival and development of R. nu.
亚洲玉米螟是一种多食性物种，能够在几种栽培和非栽培的寄主植物上发育。然而，关于这种害虫在寄主上的基本生活史信息很少。在本研究中，亚洲玉米螟幼虫不能在非 Bt 玉米、小麦、表达 Cry1Ac+Cry2Ab2 蛋白的 Bt 棉花或表达 Cry1A.105/Cry2Ab2/Cry1Ac 蛋白的 Intacta2 Xtend 大豆的叶片上存活。亚洲玉米螟在非 Bt 大豆、向日葵、油菜、巢菜、三叶草、紫花苜蓿、豆类和饲用萝卜等寄主上表现出可行的卵至成虫的生物周期（54%-66.3%），与在人工饲料上饲养的幼虫相似。此外，亚洲玉米螟幼虫不能在非 Bt 棉花上完成幼虫发育，只有 45.2%的亚洲玉米螟幼虫在食用表达 Cry1Ac 蛋白的 intacta RR2 PRO 大豆叶片时能达到蛹期。在 Cry1Ac 大豆叶片上存活的昆虫的幼虫和蛹的质量也较低（幼虫：0.104 g；蛹：0.099 g），与其他食物来源（幼虫：0.165-0.189 g；蛹：0.173-0.192 g）相比。亚洲玉米螟在 Cry1Ac 大豆叶片上的总繁殖力约为其他食物来源的 65%。与其他食物来源相比，这一事实导致其净生殖率（Ro）和内禀增长率（rm）约降低 60%。我们的研究结果表明，Cry1Ac 大豆对亚洲玉米螟的生物学参数有负面影响。非 Bt 大豆、向日葵、油菜、巢菜、三叶草、紫花苜蓿、豆类和饲用萝卜是亚洲玉米螟生存和发育的可行食物来源。
