Godói Carolina T D, Ishizuka Tamylin K, Gotardi Guilherme A, Batista Natália R F, Marques Luiz H, Santos Antônio César S, Dal Pogetto Mário H, Nowatzki Timothy, Sethi Amit, Dahmer Mark L
Corteva Agriscience, Rodovia SP 147 Km 71, Mogi Mirim 13801-540, São Paulo, Brazil.
Corteva Agriscience, 9330 Zionsville, Indianapolis, IN 46268, USA.
Insects. 2025 Apr 1;16(4):365. doi: 10.3390/insects16040365.
(Walker, 1858) and (Guenée, 1852) are important defoliating pests belonging to the Plusiinae subfamily in the Western hemisphere. is a major lepidopteran pest of soybean in the Americas, whereas, until 2021, was more restricted to the temperate regions of South America. Recently, reports of feeding on Cry1Ac soybean and occurring in tropical regions of Brazil have raised questions regarding the distribution of this species. The morphological similarity of the larvae from the two species makes it difficult to correctly identify the species in the field, which may lead to an underestimation of in Brazilian territory. This study aimed to address these questions by using a molecular approach to identify Plusiinae caterpillars throughout three seasons in non-, Cry1Ac, and Cry1Ac × Cry1F soybean fields. Here, we carried out a comprehensive spatial sampling of the primary soybean-producing regions in Brazil. The results showed that has been the main Plusiinae occurring in soybean over the last three years, and it was present in all sampled regions. For Cry1Ac and Cry1Ac × Cry1F soybeans, up to 99% of the samples collected in 2023/24 were identified as . Non- soybeans had higher variations in the proportion of the two species among the regions and across seasons, indicating that populations of and are co-occurring throughout the country. This is, to our knowledge, the most robust report assessing the distribution of and in Brazil using a molecular tool. This study provides clarification of occurrence and highlights the importance of pest monitoring from an integrated pest management perspective.
（沃克，1858年）和 （格内，1852年）是西半球夜蛾科金翅夜蛾亚科重要的食叶害虫。 是美洲大豆的主要鳞翅目害虫，而直到2021年， 更多局限于南美洲的温带地区。最近，有报道称 在巴西热带地区取食转Cry1Ac基因大豆，这引发了关于该物种分布的问题。这两个物种幼虫的形态相似性使得在田间难以正确识别物种，这可能导致巴西境内 分布的低估。本研究旨在通过分子方法解决这些问题，以识别非转基因、转Cry1Ac基因和转Cry1Ac×Cry1F基因大豆田三个季节中的金翅夜蛾亚科毛虫。在此，我们对巴西主要大豆产区进行了全面的空间采样。结果表明， 在过去三年一直是大豆田中出现的主要金翅夜蛾亚科害虫，且在所有采样地区均有发现。对于转Cry1Ac基因和转Cry1Ac×Cry1F基因大豆，2023/24年采集的样本中高达99%被鉴定为 。非转基因大豆在不同地区和季节的这两个物种比例变化更大，表明 和 在全国共同存在。据我们所知，这是使用分子工具评估 和 在巴西分布的最有力报告。本研究明确了 的发生情况，并从综合虫害管理角度突出了害虫监测的重要性。
