College of Medicine, Southwest Jiaotong University, Chengdu, Sichuan 610031, China; Key Laboratory of Advanced Technology of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan 610031, China.
Key Laboratory of Advanced Technology of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan 610031, China; Smart Industry Terminal Academy, Chengdu Technological University yibin campus, Yibin, Sichuan 644000, China.
Int J Biol Macromol. 2024 Oct;278(Pt 4):135194. doi: 10.1016/j.ijbiomac.2024.135194. Epub 2024 Sep 10.
The antimicrobial and pro-healing properties remain critical clinical objectives for skin wound management. However, the escalating problem of antibiotic overuse and the corresponding rise in bacterial resistance necessitates an urgent shift towards an antibiotic-free approach to antibacterial treatment. The quest for antimicrobial efficacy while accelerating wound healing without antibiotic treatment have emerged as innovative strategies in skin wound treatment. Here, a dual-function hydrogel with antimicrobial and enhanced tissue-healing properties was developed by utilizing cyclodextrin, ferrocene, polyethyleneimine (PEI), and Bletilla striata polysaccharide (BSP), through multiple non-covalent interactions, which can intelligently release BSP by recognizing the wound inflammatory microenvironment through the cyclodextrin-ferrocene unit. Moreover, the porosity (65 % - 85 %), Young's modulus (400 KPa - 140 KPa), and DPPH scavenge rate (18 % - 40 %) of the hydrogel are modulated by varying the BSP content. The hydrogel exhibits outstanding antibacterial properties (98.3 % reduction of Escherichia coli observed after exposure to HTFC@BSP-20 for 24 h) and favorable biocompatibility. Furthermore, in a rat full-thickness skin wound model, the dual-function hydrogel significantly accelerates wound healing, increased CD31 expression promotes vascular regeneration, reduced TNF-α express and inhibited the inflammation. This multifunctional ROS responsive hydrogel provides a new perspective for antibiotics-free treatment of skin injuries.
抗菌和促进愈合的特性仍然是皮肤伤口管理的关键临床目标。然而，抗生素过度使用的问题日益严重，细菌耐药性相应上升，这就需要迫切转向无抗生素的抗菌治疗方法。在没有抗生素治疗的情况下，寻求抗菌效果并加速伤口愈合已成为皮肤伤口治疗的创新策略。在这里，通过利用环糊精、二茂铁、聚乙烯亚胺（PEI）和白芨多糖（BSP）之间的多种非共价相互作用，开发了一种具有抗菌和增强组织愈合性能的双重功能水凝胶，该水凝胶可以通过环糊精-二茂铁单元识别伤口炎症微环境智能释放 BSP。此外，通过改变 BSP 的含量，可以调节水凝胶的孔隙率（65%-85%）、杨氏模量（400kPa-140kPa）和 DPPH 清除率（18%-40%）。水凝胶表现出优异的抗菌性能（暴露于 HTFC@BSP-20 24 小时后，大肠杆菌减少 98.3%）和良好的生物相容性。此外，在大鼠全层皮肤伤口模型中，双重功能水凝胶显著加速伤口愈合，增加 CD31 表达促进血管再生，减少 TNF-α 表达并抑制炎症。这种多功能 ROS 响应水凝胶为无抗生素治疗皮肤损伤提供了新的视角。
