Brandford White C J, Hipkiss J B
Z Parasitenkd. 1985;71(3):365-72. doi: 10.1007/BF00928338.
The influence of the phenothiazine trifluoperazine (Stellazine) on the rat tapeworm Hymenolepis diminuta was examined. The parasite was incubated in glucose-containing Krebs-Ringer media (pH 7.4) at 37 degrees C which included Ca2+ or EGTA and a range of trifluoperazine concentrations (0-2 mM). Release of soluble protein and lactate dehydrogenase activity were taken as measures of release of cytosolic components. The release of lactate dehydrogenase depended on drug concentration, maximum levels occurring at 2 mM trifluoperazine, this corresponded to 2% of the total lactate dehydrogenase present in the cestode. The effect of phenothiazines of differing lipophilicity were compared, and for trifluoperazine sulfoxide only minimal amounts of lactate dehydrogenase activity and protein were released. These values were similar to those obtained when H. diminuta was incubated in drug-free media. Our findings suggest that the integrity of the parasite is related to its calmodulin content. The potential cestocidal properties of trifluoperazine are considered.
研究了吩噻嗪类药物三氟拉嗪（司替嗪）对大鼠绦虫微小膜壳绦虫的影响。将寄生虫置于含葡萄糖的 Krebs-Ringer 培养基（pH 7.4）中，于 37℃孵育，培养基中含有 Ca2+ 或乙二醇双四乙酸（EGTA）以及一系列三氟拉嗪浓度（0 - 2 mM）。可溶性蛋白的释放和乳酸脱氢酶活性被用作细胞质成分释放的指标。乳酸脱氢酶的释放取决于药物浓度，在三氟拉嗪浓度为 2 mM 时达到最高水平，这相当于绦虫中总乳酸脱氢酶的 2%。比较了不同亲脂性吩噻嗪类药物的作用，对于三氟拉嗪亚砜，仅释放了极少量的乳酸脱氢酶活性和蛋白质。这些值与微小膜壳绦虫在无药物培养基中孵育时获得的值相似。我们的数据表明，寄生虫的完整性与其钙调蛋白含量有关。还考虑了三氟拉嗪潜在的杀绦虫特性。
