Wide L
Acta Endocrinol (Copenh). 1985 Jun;109(2):181-9. doi: 10.1530/acta.0.1090181.
The charge and charge heterogeneity of FSH, LH and TSH in 63 extracts of individual human pituitaries were investigated by use of zone electrophoresis in 0.17% agarose suspension in veronal buffer at pH 8.6 followed by hormone analyses by radioimmunoassay. The migration velocities are similar to those in free solution and directly proportional to differences in charge in the buffer. The technique gave highly reproducible results and the recovery of hormone activity after electrophoresis was about 100%. Methods for estimation of median charge, expressed as median migration rate, and of degree of charge heterogeneity are described. Each pituitary contained a sequence of different forms, estimated at 20 or more, of each of the three hormones, with minor differences in charge. The degree of charge heterogeneity within the pituitary was similar for FSH, LH and TSH. The distribution curves of radioimmunological activity in relation to migration rate were close to normality for the three glycoprotein hormones. This was in contrast to that found for Prl and GH. The merits of the electrophoretic technique are discussed.
利用在pH 8.6的巴比妥缓冲液中的0.17%琼脂糖悬浮液进行区带电泳，随后通过放射免疫测定法进行激素分析，研究了63份个体人类垂体提取物中促卵泡激素（FSH）、促黄体生成素（LH）和促甲状腺激素（TSH）的电荷及电荷异质性。迁移速度与在自由溶液中的相似，且与缓冲液中电荷差异成正比。该技术给出了高度可重复的结果，电泳后激素活性的回收率约为100%。描述了以中位迁移率表示的中位电荷及电荷异质性程度的估计方法。每个垂体含有三种激素中每种激素的一系列不同形式，估计有20种或更多，电荷存在微小差异。垂体中FSH、LH和TSH的电荷异质性程度相似。三种糖蛋白激素的放射免疫活性相对于迁移率的分布曲线接近正态分布。这与催乳素（Prl）和生长激素（GH）的情况相反。讨论了电泳技术的优点。
