Björndahl L, Kvist U
Acta Physiol Scand. 1985 Jun;124(2):189-94. doi: 10.1111/j.1748-1716.1985.tb07651.x.
Chromatin decondensation of human ejaculated spermatozoa was studied in vitro, at various points of time after ejaculation, by sperm exposure to the detergent sodium dodecyl sulphate (SDS) containing zinc chelating EDTA. Within 5 min after ejaculation EDTA revealed a capacity for decondensation in 90% of the spermatozoa. This sperm capacity decreased rapidly upon storage. The results support the concept that the capacity to decondense is a normal property of freshly ejaculated spermatozoa and that this property may be rapidly lost. The loss is most probably due to an inability of thiol groups to take part in a thiol-disulphide exchange in the sperm chromatin. A loss of functional thiols may hinder a capacity for chromatin decondensation inherent to the spermatozoon. A loss of thiols due to oxidation, that is, surplus S-S bridge formation, may also delay hypothetical extrinsic S-S cleaving factors in the ooplasm. In either case, the complete and non-delayed sperm chromatin decondensation in the ovum may be hindered. This may result in the abnormal embryonic development observed in ova fertilized with aged spermatozoa.
通过将精子暴露于含有锌螯合剂乙二胺四乙酸（EDTA）的去污剂十二烷基硫酸钠（SDS）中，在体外对射精后不同时间点的人类射出精子的染色质解聚进行了研究。射精后5分钟内，EDTA显示90%的精子具有解聚能力。这种精子能力在储存时迅速下降。结果支持这样的概念，即解聚能力是新鲜射出精子的正常特性，并且这种特性可能会迅速丧失。这种丧失很可能是由于硫醇基团无法参与精子染色质中的硫醇-二硫键交换。功能性硫醇的丧失可能会阻碍精子固有的染色质解聚能力。由于氧化导致的硫醇丧失，即形成过多的S-S桥，也可能会延迟卵细胞质中假设的外在S-S裂解因子。在任何一种情况下，卵子中精子染色质的完全且无延迟的解聚都可能受到阻碍。这可能导致在用老化精子受精的卵子中观察到异常胚胎发育。
