Wang Xinqing, Chen Shuo, Chen Xiaolei, Wu Juan, Huang Zhenhua, Wang Jing, Chen Fangping, Liu Changsheng
Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai, 200237, PR China.
Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai, 200237, PR China.
Bioact Mater. 2024 Aug 24;41:577-596. doi: 10.1016/j.bioactmat.2024.07.018. eCollection 2024 Nov.
Peripheral nervous system (PNS) injuries often lead to significant sensory and motor impairments. Traditional artificial nerve conduits, lacking anisotropic structures, have been associated with prolonged repair time and failures in nerve regeneration. This study aimed to address these challenges by developing a novel approach for rapid repair of peripheral nerve injuries (PNI). A 3D oriented fibers scaffold featuring distinct radial (RFs) and longitudinal (LFs) fibers orientations was engineered using coaxial electrospinning and gas directional foaming techniques. This scaffold was then integrated with a shape memory conduit to form a directional multi-channel nerve conduit with micro/nanostructures. The results revealed that the grooved surface of the fibers significantly improved cellular directional guidance, effectively facilitating the migration of SCs from the periphery towards the center and from the base to the apex of the scaffold. In a rat model with a 10 mm nerve defect, the ND-PLATMC/LF ND-PCL scaffold significantly enhanced nerve regeneration and motor function recovery within 4 weeks. These results suggest the potential of this innovative scaffold for efficient repair of the nerve injuries.
外周神经系统（PNS）损伤常导致严重的感觉和运动障碍。传统的人工神经导管缺乏各向异性结构，与修复时间延长和神经再生失败有关。本研究旨在通过开发一种快速修复周围神经损伤（PNI）的新方法来应对这些挑战。采用同轴电纺丝和气体定向发泡技术设计了一种具有不同径向（RFs）和纵向（LFs）纤维取向的三维定向纤维支架。然后将该支架与形状记忆导管集成，形成具有微/纳米结构的定向多通道神经导管。结果表明，纤维的沟槽表面显著改善了细胞的定向引导，有效地促进了雪旺细胞从外周向中心以及从支架底部向顶部的迁移。在一个有10毫米神经缺损的大鼠模型中，ND-PLATMC/LF ND-PCL支架在4周内显著增强了神经再生和运动功能恢复。这些结果表明这种创新支架在有效修复神经损伤方面具有潜力。
