Meyer M E
Am J Vet Res. 1985 Apr;46(4):902-4.
One hundred isolates of Brucella abortus, which were recovered from bovine and human tissues or fluids, were identified as strain 19 by conventional bacteriologic methods. Each isolate was examined using a Warburg respirometer to determine oxidative rates on substrates of D- and L-alanine, L-glutamic acid, d(+)-galactose, D-ribose, and i-erythritol. These results were compared with those of repository (seed) cultures of strain 19 used for making antigens and vaccines. Except on the substrate of i-erythritol, each of the 100 isolates oxidized these substrates with rates different from the repository cultures and indistinguishable from those of field strains of B abortus. Thus, oxidatively, i-erythritol was the only substrate useful to help distinguish between strain 19 and virulent strains of B abortus biotype 1.
从牛和人的组织或体液中分离出的100株流产布鲁氏菌，通过传统细菌学方法鉴定为19株。使用瓦氏呼吸计对每株分离菌进行检测，以确定其对D-丙氨酸、L-丙氨酸、L-谷氨酸、D-(+)-半乳糖、D-核糖和赤藓醇等底物的氧化速率。将这些结果与用于制备抗原和疫苗的19株菌种保藏（种子）培养物的结果进行比较。除了在赤藓醇底物上，这100株分离菌中的每一株对这些底物的氧化速率都与菌种保藏培养物不同，且与流产布鲁氏菌生物型1的野外菌株无法区分。因此，从氧化方面来看，赤藓醇是唯一有助于区分19株菌和流产布鲁氏菌生物型1强毒株的底物。
