羊瘙痒病病原体对小鼠神经胶质细胞和成神经细胞瘤细胞的体外感染效应。

Effects of in vitro infection of mouse glial and neuroblastoma cells with the scrapie agent.

作者信息

Markovits P, Mutel V, Dianoux L, Launay J M, Haimart M, Dormont D, Court L, Cathala F

出版信息

Ann Rech Vet. 1985;16(1):111-9.

Abstract

Mouse glial and neuroblastoma cells were infected with the mouse adapted strains C506 and 139A of scrapie agent. Lysates of the in vitro infected cells (from the 3rd to the 16th passage) intracerebrally inoculated into CD-1 mice, caused the development of a neurological disease, with characteristic signs of scrapie. Morphological changes in scrapie-infected neural cells were observed after about fifteen in vitro passages. In liquid medium, the cloning efficiency of these cells increased. They acquired the capacity to form large tridimensional colonies in agar. Heating the infectious brain extracts at 60 and 80 degrees C for 30 minutes did not inhibit these changes thus showing the involvement of the thermoresistant scrapie agent. Supernatants of scrapie-infected glial cells promoted colony formation in liquid medium with different types of normal cells. Analysis of supernatants of scrapie-infected mouse neuroblastoma cells showed a profound modification of neurotransmitter metabolism.

摘要

将小鼠适应株C506和139A瘙痒病病原体感染小鼠神经胶质细胞和成神经细胞瘤细胞。将体外感染细胞（第3代至第16代）的裂解物脑内接种到CD-1小鼠中，引发了一种神经系统疾病，具有瘙痒病的特征性体征。在大约15次体外传代后，观察到瘙痒病感染神经细胞的形态学变化。在液体培养基中，这些细胞的克隆效率增加。它们获得了在琼脂中形成大型三维集落的能力。将感染性脑提取物在60℃和80℃加热30分钟并不抑制这些变化，从而表明耐热性瘙痒病病原体的参与。瘙痒病感染的神经胶质细胞的上清液促进了不同类型正常细胞在液体培养基中的集落形成。对瘙痒病感染的小鼠成神经细胞瘤细胞的上清液分析显示神经递质代谢发生了深刻改变。