Department of Pathology, School of Medicine, Iran University of Medical Sciences.
Oncopathology Research Center, Iran University of Medical Sciences.
Appl Immunohistochem Mol Morphol. 2024 Oct 1;32(9):425-435. doi: 10.1097/PAI.0000000000001220. Epub 2024 Sep 11.
Talin-1 is one of the major scaffold proteins in focal adhesions playing a vital role in cell migration, metastasis, and cancer progression. Although studies regarding the importance of Talin-1 in cancer have rapidly developed, its prognostic and diagnostic value still remain unsatisfying in pancreatic cancer (PC). Therefore, the present study aims to investigate the expression, clinical significance, as well as the prognostic and diagnostic value of Talin-1 in different types of PC. Bioinformatic analysis was applied to determine the clinical importance and biological role of Talin-1 expression in PC tumors and the normal adjacent samples. The expression patterns, clinical significance, prognosis, and diagnosis value of Talin-1 were evaluated in tissue microarrays (TMAs) of 190 PC samples including 170 pancreatic ductal adenocarcinoma (PDAC), and 20 pancreatic neuroendocrine tumors (PNET), along with 24 adjacent normal tissues using immunohistochemistry (IHC). The results indicated that the expression of Talin-1 was upregulated in tumor cells compared with adjacent normal tissues. A statistically significant association was observed between the higher cytoplasmic expression of Talin-1 and lower histologic grade ( P <0.001) in PDAC samples. Further, our findings indicated an inverse significant correlation between cytoplasmic expression of Talin-1 and recurrence ( P =0.014) in PNET samples. No significant association was observed between the cytoplasmic expression of Talin-1 and survival outcomes as well as diagnostic accuracy. In conclusion, our observations demonstrated that a higher cytoplasmic level of Talin-1 protein was significantly associated with less aggressive tumor behaviors in PC samples. Nevertheless, further investigations are required to explore the prognostic plus diagnostic value, and mechanism of action of Talin-1 in pancreatic cancer.
Talin-1 是黏着斑中的主要支架蛋白之一，在细胞迁移、转移和癌症进展中发挥着至关重要的作用。尽管关于 Talin-1 在癌症中的重要性的研究已经迅速发展，但它在胰腺癌（PC）中的预后和诊断价值仍然不尽如人意。因此，本研究旨在探讨 Talin-1 在不同类型 PC 中的表达、临床意义以及预后和诊断价值。生物信息学分析用于确定 Talin-1 在 PC 肿瘤和正常相邻样本中的临床重要性和生物学作用。使用免疫组织化学（IHC）在 190 个 PC 样本的组织微阵列（TMA）中评估了 Talin-1 的表达模式、临床意义、预后和诊断价值，其中包括 170 个胰腺导管腺癌（PDAC）和 20 个胰腺神经内分泌肿瘤（PNET），以及 24 个相邻正常组织。结果表明，与相邻正常组织相比，肿瘤细胞中 Talin-1 的表达上调。在 PDAC 样本中，Talin-1 的细胞质高表达与组织学分级较低呈显著相关（P<0.001）。此外，我们的研究结果表明，在 PNET 样本中，Talin-1 的细胞质表达与复发呈负相关（P=0.014）。Talin-1 的细胞质表达与生存结局和诊断准确性之间没有显著相关性。总之，我们的观察表明，PC 样本中 Talin-1 蛋白的细胞质水平较高与肿瘤行为侵袭性较弱显著相关。然而，需要进一步研究来探讨 Talin-1 在胰腺癌中的预后和诊断价值及其作用机制。
