经皮耳迷走神经刺激（taVNS）在清醒小鼠中的可行性和有效性。
Feasibility and effectiveness of transcutaneous auricular vagus nerve stimulation (taVNS) in awake mice.
机构信息
Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical University, Xuzhou, Jiangsu, China.
Jiangsu Province Key Laboratory of Anesthesia and Analgesia Application Technology, Xuzhou Medical University, Xuzhou, Jiangsu, China.
出版信息
CNS Neurosci Ther. 2024 Sep;30(9):e70043. doi: 10.1111/cns.70043.
AIMS
Transcutaneous auricular vagus nerve stimulation (taVNS) is widely used to treat a variety of disorders because it is noninvasive, safe, and well tolerated by awake patients. However, long-term and repetitive taVNS is difficult to achieve in awake mice. Therefore, developing a new taVNS method that fully mimics the method used in clinical settings and is well-tolerated by awake mice is greatly important for generalizing research findings related to the effects of taVNS. The study aimed to develop a new taVNS device for use in awake mice and to test its reliability and effectiveness.
METHODS
We demonstrated the reliability of this taVNS device through retrograde neurotropic pseudorabies virus (PRV) tracing and evaluated its effectiveness through morphological analysis. After 3 weeks of taVNS application, the open field test (OFT) and elevated plus maze (EPM) were used to evaluate anxiety-like behaviors, and the Y-maze test and novel object recognition test (NORT) were used to evaluate recognition memory behaviors, respectively.
RESULTS
We found that repetitive taVNS was well tolerated by awake mice, had no effect on anxiety-like behaviors, and significantly improved memory.
CONCLUSION
Our findings suggest that this new taVNS device for repetitive stimulation of awake mice is safe, tolerable, and effective.
目的
经皮耳迷走神经刺激（taVNS）因其非侵入性、安全性和清醒患者良好耐受性而被广泛用于治疗各种疾病。然而，在清醒小鼠中实现长期和重复的 taVNS 较为困难。因此，开发一种新的 taVNS 方法，该方法充分模拟临床应用的方法，且清醒小鼠能够很好地耐受，对于推广与 taVNS 效果相关的研究结果非常重要。本研究旨在开发一种用于清醒小鼠的新型 taVNS 设备，并测试其可靠性和有效性。
方法
我们通过逆行神经嗜性伪狂犬病毒（PRV）示踪证明了这种 taVNS 设备的可靠性，并通过形态分析评估了其有效性。在 taVNS 应用 3 周后，使用旷场试验（OFT）和高架十字迷宫（EPM）分别评估焦虑样行为，使用 Y 迷宫测试和新颖物体识别测试（NORT）分别评估识别记忆行为。
结果
我们发现，重复 taVNS 能够被清醒小鼠很好地耐受，对焦虑样行为没有影响，且显著改善了记忆。
结论
我们的研究结果表明，这种用于清醒小鼠重复刺激的新型 taVNS 设备是安全、耐受和有效的。
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