Maeda T, Iwanaga T, Fujita T, Kobayashi S
Arch Histol Jpn. 1985 Feb;48(1):123-9. doi: 10.1679/aohc.48.123.
Human dental pulp was investigated by an immunofluorescence method using antisera against neurofilament protein (NFP) and glia-specific S-100 protein. Nerve fibers coursing through the pulp were selectively stained with the anti-NFP serum, and their Schwann sheath with the S-100 antiserum. In addition to a definitive demonstration of the subodontoblastic nerve plexus, we found another hitherto unknown nerve plexus composed of delicate fibers at the base of the odontoblastic layer. This study shows that immunohisto chemical techniques using antisera against nervous system-specific proteins are superior to previous silver impregnation methods, due to their specificity and constancy in reaction.
采用抗神经丝蛋白（NFP）和神经胶质特异性S - 100蛋白的抗血清，通过免疫荧光法对人牙髓进行了研究。穿过牙髓的神经纤维被抗NFP血清选择性染色，其施万细胞鞘被S - 100抗血清染色。除了明确显示成牙本质细胞下神经丛外，我们还在成牙本质细胞层底部发现了另一个由纤细纤维组成的迄今未知的神经丛。本研究表明，使用针对神经系统特异性蛋白的抗血清的免疫组织化学技术，由于其反应的特异性和稳定性，优于以前的银浸染法。
