对网织红细胞脂氧合酶的圆二色性观察。
Circular dichroism observations on the lipoxygenase from reticulocytes.
作者信息
Ludwig P, Tordi M G, Colosimo A
出版信息
Biochim Biophys Acta. 1985 Aug 8;830(2):136-9. doi: 10.1016/0167-4838(85)90020-2.
CD spectra of reticulocyte lipoxygenase were recorded at various times during aerobic incubation, which leads to loss of catalytic activity, and after inactivation by the reaction product, 13-LS-hydroperoxylinoleic acid. Neither process showed any correlation with a decrease in the alpha-helix content, which was only observed over much longer times. It is concluded that inactivation of reticulocyte lipoxygenase by the suicidal reaction with 13-LS-hydroperoxylinoleic acid is not accompanied by gross structural alterations.
在需氧孵育过程中，于不同时间记录网织红细胞脂氧合酶的圆二色光谱，该过程会导致催化活性丧失，并且在被反应产物13-LS-氢过氧亚油酸灭活后也进行了记录。这两个过程均未显示出与α-螺旋含量降低有任何相关性，α-螺旋含量的降低仅在更长时间内才会观察到。得出的结论是，网织红细胞脂氧合酶与13-LS-氢过氧亚油酸发生自杀反应而失活，并未伴随明显的结构改变。
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