Department of Convergence IT Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Nam-Gu, Pohang, 37673, Republic of Korea.
Department of Materials Science and Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Nam-Gu, Pohang, 37673, Republic of Korea.
Biomaterials. 2025 Mar;314:122806. doi: 10.1016/j.biomaterials.2024.122806. Epub 2024 Sep 2.
Assessing the transepithelial resistance to ion flow in the presence of an electric field enables the evaluation of the integrity of the epithelial cell layer. In this study, we introduce an organic electrochemical transistor (OECT) interfaced with a 3D living tissue, designed to monitor the electrical resistance of cellular barriers in real-time. We have developed a non-invasive, tissue-sensing platform by integrating an inkjet-printed large-area OECT with a 3D-bioprinted multilayered airway tissue. This unique configuration enables the evaluation of epithelial barrier integrity through the dynamic response capabilities of the OECT. Our system effectively tracks the formation and integrity of 3D-printed airway tissues in both liquid-liquid and air-liquid interface culture environments. Furthermore, we successfully quantified the degradation of barrier function due to influenza A (H1N1) viral infection and the dose-dependent efficacy of oseltamivir (Tamiflu®) in mitigating this degradation. The tissue-electronic platform offers a non-invasive and label-free method for real-time monitoring of 3D artificial tissue barriers, without disturbing the cellular biology. It holds the potential for further applications in monitoring the structures and functions of 3D tissues and organs, significantly contributing to the advancement of personalized medicine.
评估存在电场时离子流的跨上皮电阻能够评估上皮细胞层的完整性。在这项研究中，我们引入了一种与 3D 活体组织接口的有机电化学晶体管 (OECT)，旨在实时监测细胞屏障的电阻。我们通过将喷墨打印的大面积 OECT 与 3D 生物打印的多层气道组织集成，开发了一种非侵入性、组织感应平台。这种独特的配置通过 OECT 的动态响应能力来评估上皮屏障的完整性。我们的系统能够有效地跟踪液体-液体和空气-液体界面培养环境中 3D 打印气道组织的形成和完整性。此外，我们成功地量化了由于流感 A（H1N1）病毒感染导致的屏障功能下降，以及奥司他韦（达菲®）在减轻这种下降方面的剂量依赖性疗效。该组织-电子平台提供了一种非侵入性和无标记的方法来实时监测 3D 人工组织屏障，而不会干扰细胞生物学。它有可能进一步应用于监测 3D 组织和器官的结构和功能，为个性化医疗的发展做出重大贡献。
