Max Planck Institute for Polymer Research, Ackermannweg 10, 55128, Mainz, Germany.
Department of Information Engineering, University of Brescia, Via Branze 38, 25123, Brescia, Italy.
Adv Healthc Mater. 2019 Aug;8(16):e1900128. doi: 10.1002/adhm.201900128. Epub 2019 Jul 18.
The integrity of CaCo-2 cell barriers is investigated by organic electrochemical transistors (OECTs) in a current-driven configuration. Ion transport through cellular barriers via the paracellular pathway is modulated by tight junctions between adjacent cells. Rupturing its integrity by H O is monitored by the change of the output voltage in the transfer characteristics. It is demonstrated that by operating the OECT in a current-driven configuration, the sensitive and temporal resolution for monitoring the cell barrier integrity is strongly enhanced as compared to the OECT transient response measurement. As a result, current-driven OECTs are useful tools to assess dynamic and critical changes in tight junctions, relevant for clinical applications as drug targeting and screening.
通过在电流驱动模式下的有机电化学晶体管（OECT）研究 CaCo-2 细胞屏障的完整性。离子通过细胞旁途径穿过细胞屏障的运输通过相邻细胞之间的紧密连接进行调节。通过 H2O 的破坏来监测其完整性，这可以通过输出电压在转移特性中的变化来监测。结果表明，与 OECT 瞬态响应测量相比，通过将 OECT 操作在电流驱动模式下，可以强烈增强对细胞屏障完整性进行敏感和时间分辨监测的能力。因此，电流驱动的 OECT 是评估与临床应用（如药物靶向和筛选）相关的紧密连接的动态和关键变化的有用工具。
