The College of Food Science, Shenyang Agricultural University, Shenyang 110866, China; Institute of Food Science and Technology, Chinese Academy of Agriculture Sciences, Key Laboratory of Agro-Products Quality & Safety Harvest, Storage, Transportation, Management and Control, Ministry of Agriculture and Rural Affairs, Beijing 100193, China.
Institute of Food Science and Technology, Chinese Academy of Agriculture Sciences, Key Laboratory of Agro-Products Quality & Safety Harvest, Storage, Transportation, Management and Control, Ministry of Agriculture and Rural Affairs, Beijing 100193, China.
Food Chem. 2025 Jan 15;463(Pt 1):141196. doi: 10.1016/j.foodchem.2024.141196. Epub 2024 Sep 8.
Soup is an important presence in diet, but its absorption and transport mechanism by the human body remains unclear. In this study, Caco-2 intact cell and monolayer cell models were constructed to simulate small intestine absorption on colloidal nanoparticles (CNPs) isolated from lamb soup. The intracellular localization of CNPs was viewed by laser confocal microscopy (LSCM). CNPs uptake and release pathways were explored by differences in CNPs concentrations in 5 endocytosis inhibitor models and 4 exocytosis inhibitor models. Results indicated that CNPs endocytosis by Caco-2 cells was restrained by Nystatin and Cytochalasin D, with exocytosis being inhibited by Nocodazole and Monensin. Therefore, the major absorption pathways for CNPs were caveolin-dependent endocytosis, macropinocytosis and phagocytosis. The major transport pathways were microtubule-vesicle-mediated protein transport to the membrane and transportation between the Golgi apparatus and membrane. This study may provide theoretical support for the transport mechanism of soup products in the small intestine.
汤在饮食中占有重要地位，但人体对其的吸收和转运机制尚不清楚。本研究构建了 Caco-2 完整细胞和单层细胞模型，以模拟从小羊汤中分离出的胶体纳米颗粒（CNPs）对小肠的吸收。通过激光共聚焦显微镜（LSCM）观察 CNPs 的细胞内定位。通过 5 种内吞抑制剂模型和 4 种外排抑制剂模型中 CNPs 浓度的差异，探讨了 CNPs 的摄取和释放途径。结果表明，CNPs 被 Caco-2 细胞的内吞作用受到制霉菌素和细胞松弛素 D 的抑制，而外排作用则受到诺考达唑和莫能菌素的抑制。因此，CNPs 的主要吸收途径是网格蛋白依赖的内吞作用、巨胞饮作用和吞噬作用。主要的转运途径是微管-囊泡介导的蛋白质向膜的转运以及高尔基体和膜之间的转运。本研究可为汤类产品在小肠中的转运机制提供理论支持。
