Université de Lorraine, INRAE, UMR1121 Laboratoire Agronomie et Environnement (LAE), F-54000, Nancy, France.
EA 2106, Biomolécules et Biotechnologies Végétales (BBV), Université de Tours, Tours, France.
Phytochemistry. 2025 Jan;229:114271. doi: 10.1016/j.phytochem.2024.114271. Epub 2024 Sep 12.
Phenolamides are specialized metabolites widely distributed in the plant kingdom. Their structure is composed by the association of hydroxycinnamic acid derivatives to mono-/poly-amine through an amination catalyzed by N-hydroxycinnamoyltransferases enzymes. Tomato plants accumulate putrescine-derived phenolamides in their vegetative parts. Recently, two first genes coding for putrescine-hydroxycinnamoyltransferase (PHT, Solyc11g071470 and Solyc11g071480) were identified in tomato and demonstrated to control the leaf accumulation of caffeoylputrescine in response to leafminer infestation. In this study, two additional genes (Solyc06g074710 and Solyc11g066640) were functionally characterized as new tomato PHT. The substrate specificity and the expression pattern in planta were determined for the four tomato PHT. Taken together the results give a comprehensive view of the control of the putrescine-derived phenolamide accumulation in tomato plant through the biochemical specificity and the spatial expression of this small family of PHT.
苯甲酰胺类化合物是广泛分布于植物界的一类特殊代谢物。它们的结构由羟基肉桂酸衍生物通过 N-羟基肉桂酰转移酶的氨基化作用与单/多胺结合而成。番茄植物在其营养部分积累腐胺衍生的苯甲酰胺类化合物。最近，在番茄中鉴定出两个编码腐胺-羟基肉桂酰转移酶（PHT，Solyc11g071470 和 Solyc11g071480）的首个基因，并证明它们可以控制叶片中叶蝉侵害时的咖啡酰腐胺积累。在这项研究中，另外两个基因（Solyc06g074710 和 Solyc11g066640）被功能表征为新的番茄 PHT。对四个番茄 PHT 的底物特异性和体内表达模式进行了测定。综合这些结果，全面了解了通过这个小的 PHT 家族的生化特异性和空间表达来控制番茄植物中腐胺衍生的苯甲酰胺类化合物的积累。
