胺化木质素提高了对甲苯磺酸处理的纤维素底物的酶水解效率。
Aminated lignin improved enzymatic hydrolysis of cellulosic substrate treated by p-toluenesulfonic acid.
机构信息
National Engineering Research Center of Rice and Byproduct Deep Processing, College of Food Science and Engineering, Central South University of Forestry and Technology, 498 South Shaoshan Rd., Tianxin District, Changsha 410004, China; Faculty of Food Science and Engineering, Kunming University of Science and Technology, 727 South Jingming Rd., Chenggong District, Kunming 650500, China.
Faculty of Food Science and Engineering, Kunming University of Science and Technology, 727 South Jingming Rd., Chenggong District, Kunming 650500, China.
出版信息
J Biotechnol. 2024 Nov 20;395:44-52. doi: 10.1016/j.jbiotec.2024.09.004. Epub 2024 Sep 12.
Lignin can affect the enzymatic hydrolysis efficiency of lignocellulose. In this study, the lignin isolated from sugarcane bagasse (SCB) pretreated with p-toluenesulfonic acid (PL) was firstly aminated, and then the effects of PL and aminated PL (APL) on the bagasse enzymatic hydrolysis efficiency (EHE) were investigated. The results showed that the addition of PL and APL promoted the EHE, and EHE with APL (73.82 %) was higher than PL (51.39 %). To explore the reason, the data were further analyzed including cellulase adsorption capacity, enzyme activity, cellulase-lignin interaction, and molecular docking. It was found that APL adsorbed more cellulase (27.83 mg protein/g lignin) than PL (4.96 mg protein/g lignin), resulting from the greater interaction force and lower binding free energy between APL and cellulase. The addition of APL more remarkably enhanced the cellobiohydrolase and endoglucanase activities than PL due to more effectively inducing cellulase conformation optimization.
木质素会影响木质纤维素的酶解效率。在这项研究中，首先对经对甲苯磺酸（PL）预处理的甘蔗渣（SCB）中的木质素进行了氨化，然后研究了 PL 和氨化 PL（APL）对蔗渣酶解效率（EHE）的影响。结果表明，添加 PL 和 APL 均能促进 EHE，且 APL 的 EHE（73.82%）高于 PL（51.39%）。为了探究原因，进一步对包括纤维素酶吸附能力、酶活性、纤维素酶-木质素相互作用和分子对接在内的数据进行了分析。结果发现，APL 吸附的纤维素酶（27.83 mg 蛋白/g 木质素）多于 PL（4.96 mg 蛋白/g 木质素），这是由于 APL 与纤维素酶之间的相互作用力更大，结合自由能更低。APL 比 PL 更显著地提高了纤维二糖水解酶和内切葡聚糖酶的活性，这是因为 APL 更有效地诱导了纤维素酶构象的优化。
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