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微管生长驱动的内膜运输调节拟南芥气孔运动。

Endomembrane trafficking driven by microtubule growth regulates stomatal movement in Arabidopsis.

机构信息

State Key Laboratory of Plant Environmental Resilience, College of Biological Sciences, China Agricultural University, Beijing, China.

Joint Laboratory for International Cooperation in Crop Molecular Breeding, Ministry of Education, China Agricultural University, Beijing, China.

出版信息

Nat Commun. 2024 Sep 11;15(1):7967. doi: 10.1038/s41467-024-52338-x.

DOI:10.1038/s41467-024-52338-x
PMID:39261498
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11391047/
Abstract

Microtubule-based vesicle trafficking usually relies upon kinesin and dynein motors and few reports describe microtubule polymerisation driving directional vesicle trafficking. Here we show that Arabidopsis END BINDING1b (EB1b), a microtubule plus-end binding protein, directly interacts with SYP121, a SNARE protein that mediates the trafficking of the K channel KAT1 and its distribution to the plasma membrane (PM) in Arabidopsis guard cells. Knockout of AtEB1b and its homologous proteins results in a modest but significant change in the distribution of KAT1 and SYP121 in guard cells and consequently delays light-induced stomatal opening. Live-cell imaging reveals that a portion of SYP121-associated endomembrane compartments co-localise with AtEB1b at the growing ends of microtubules, trafficking along with the growth of microtubules for targeting to the PM. Our study reveals a mechanism of vesicle trafficking driven by microtubule growth, which is involved in the redistribution of PM proteins to modulate guard cell movement.

摘要

基于微管的囊泡运输通常依赖于驱动蛋白和动力蛋白，很少有报道描述微管聚合驱动囊泡的定向运输。在这里，我们发现拟南芥末端结合蛋白 1b（EB1b），一种微管正端结合蛋白，直接与 SYP121 相互作用，SYP121 是一种 SNARE 蛋白，介导 K 通道 KAT1 的运输及其在拟南芥保卫细胞中的分布到质膜（PM）。AtEB1b 和其同源蛋白的敲除导致 KAT1 和 SYP121 在保卫细胞中的分布有轻微但显著的变化，从而延迟了光诱导的气孔开放。活细胞成像显示，一部分与 SYP121 相关的内膜隔室与生长中的微管末端的 AtEB1b 共定位，沿微管的生长运输，以靶向 PM。我们的研究揭示了一种由微管生长驱动的囊泡运输的机制，该机制涉及到 PM 蛋白的重新分布，以调节保卫细胞的运动。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4cf/11391047/4e70d61aaee6/41467_2024_52338_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4cf/11391047/4a3e7b7c5a25/41467_2024_52338_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4cf/11391047/c1ce7f8f217c/41467_2024_52338_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4cf/11391047/674c34c13a49/41467_2024_52338_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4cf/11391047/1aa255203443/41467_2024_52338_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4cf/11391047/607c5e2c4ce5/41467_2024_52338_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4cf/11391047/9d975e9212b4/41467_2024_52338_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4cf/11391047/4e70d61aaee6/41467_2024_52338_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4cf/11391047/4a3e7b7c5a25/41467_2024_52338_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4cf/11391047/c1ce7f8f217c/41467_2024_52338_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4cf/11391047/674c34c13a49/41467_2024_52338_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4cf/11391047/1aa255203443/41467_2024_52338_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4cf/11391047/607c5e2c4ce5/41467_2024_52338_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4cf/11391047/9d975e9212b4/41467_2024_52338_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4cf/11391047/4e70d61aaee6/41467_2024_52338_Fig7_HTML.jpg

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Arabidopsis SYP121 acts as an ROP2 effector in the regulation of root hair tip growth.拟南芥 SYP121 作为 ROP2 效应物在调控根毛尖端生长中起作用。
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Arabidopsis myosin XIK interacts with the exocyst complex to facilitate vesicle tethering during exocytosis.拟南芥肌球蛋白 XIK 与外泌体复合物相互作用，以促进胞吐作用期间囊泡的连接。
Plant Cell. 2021 Aug 13;33(7):2454-2478. doi: 10.1093/plcell/koab116.
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