Singh Shriti, Gehlot Sangeeta, Agrawal Neeraj Kumar, Singh Girish, Singh Devshree, Kaur Prabhjot, Singh Santosh Kumar, Singh Rajesh
Banaras Hindu University, Institute of Medical Sciences, Faculty of Ayurveda, Department of Kriya Sharir, Varanasi, 221005, Uttar Pradesh, India.
Banaras Hindu University, Institute of Medical Sciences, Department of Endocrinology and Metabolism, Varanasi, 221005, Uttar Pradesh, India.
J Tradit Complement Med. 2024 Jan 11;14(5):494-500. doi: 10.1016/j.jtcme.2024.01.004. eCollection 2024 Sep.
In Ayurveda, every individual is believed to possess a unique entity known as which distinguishes them from others physically, physiologically, and psychologically. This entity also determines an individual's response to a particular stimulus, and it is believed that such responses are not solely determined by genetics. The present research aims to validate the Ayurvedic concept of from a modern molecular perspective to strengthen the personalized and precise treatment approach. A study was conducted to investigate the role of the KCNJ11gene in the susceptibility of individuals to type 2 diabetes mellitus (T2DM) with their metabolic status. The research involved allele mining on three major groups - , and in 112 patients with T2DM and 112 healthy individuals. The KCNJ11 gene, responsible for insulin secretion membrane pore formation, was analyzed to determine the susceptibility of different types to T2DM. The MutPred tool predicted the molecular cause of disease-related amino acid substitution. According to the study, only and were diagnosed with diabetes, while all three types were present in the control group of healthy individuals. A protein model was prepared, and the changes resulting from mutations were observed for each group in their protein sequence, both as synonymous and non-synonymous mutations. Ultimately, these changes contributed to the manifestation of T2DM. Based on the findings, it appears that groups may experience changes in protein function due to nonsynonymous mutations and differences in amino acids at the protein level.
在阿育吠陀医学中，每个人都被认为拥有一种独特的实体，称为“体质”，它在身体、生理和心理上使他们与其他人区分开来。这个实体还决定了个体对特定刺激的反应，并且人们认为这种反应不仅仅由基因决定。本研究旨在从现代分子角度验证阿育吠陀医学的“体质”概念，以加强个性化和精准的治疗方法。进行了一项研究，调查KCNJ11基因在个体对2型糖尿病（T2DM）易感性及其代谢状态中的作用。该研究对112例T2DM患者和112例健康个体的三个主要“体质”类型——瓦塔、皮塔和卡法进行了等位基因挖掘。对负责胰岛素分泌膜孔形成的KCNJ11基因进行分析，以确定不同“体质”类型对T2DM的易感性。MutPred工具预测了与疾病相关的氨基酸替代的分子原因。根据研究，只有瓦塔和皮塔被诊断患有糖尿病，而所有三种“体质”类型都出现在健康个体的对照组中。制备了蛋白质模型，并观察了每组在其蛋白质序列中由突变引起的变化，包括同义突变和非同义突变。最终，这些变化导致了T2DM的表现。基于这些发现，似乎“体质”类型可能由于非同义突变和蛋白质水平上氨基酸的差异而经历蛋白质功能的变化。
