Sun Peiwen, Lv Feifei, Yang Yun, Hou Wencheng, Xiao Mengjun, Gao Zhihui, Xu Yanhong, Wei Jianhe
Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education & National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100193, China.
Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine & Key Laboratory of State Administration of Traditional Chinese Medicine for Agarwood Sustainable Utilization, Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou, 570311, China.
Heliyon. 2024 Aug 13;10(16):e35874. doi: 10.1016/j.heliyon.2024.e35874. eCollection 2024 Aug 30.
Agarwood is a rare and valuable heartwood derived from in China. Compared with ordinary germplasm, Chi-Nan, a special germplasm of , has a better agarwood-producing capacity. However, the mechanisms underlying their different qualities remain poorly characterized. Here, a comparative transcriptome analysis of Chi-Nan and ordinary was carried out to investigate the wound responses of both germplasms. A total of 198.19 Gb of clean data were obtained with an average of 6.61 Gb of clean reads for each sample. By comparing with their control groups, more differentially expressed genes (DEGs) were observed in Chi-Nan germplasm. Kyoto Encyclopedia of Genes and Genomes (KEGG) and expression profile analysis suggested that Chi-Nan possesses a stronger ability to respond to wounding. Furthermore, the enrichment of biosynthetic pathways related to sesquiterpenes and 2-(2-phenylethyl) chromones (PECs) were more significant in Chi-Nan than in ordinary germplasm, and related genes showed significantly higher up-regulation in Chi-Nan after wounding. Sixteen candidate genes presumably involved in biosynthesis of agarwood components were identified and found to exhibit higher up-regulation in Chi-Nan than in ordinary germplasm in response to wounding. Overall, these results are helpful in explaining reasons for the higher agarwood-producing properties of Chi-Nan, and contribute to a further understanding of the mechanism of agarwood formation in .
沉香是一种源自中国的珍稀且珍贵的沉香木。与普通种质相比，奇楠作为一种特殊的沉香种质，具有更强的产香能力。然而，它们品质差异背后的机制仍不清楚。在此，对奇楠和普通沉香进行了比较转录组分析，以研究这两种种质的伤口反应。共获得198.19Gb的 clean 数据，每个样本平均有6.61Gb的 clean reads。通过与对照组比较，在奇楠种质中观察到更多差异表达基因（DEGs）。京都基因与基因组百科全书（KEGG）和表达谱分析表明，奇楠具有更强的伤口反应能力。此外，与倍半萜和2-(2-苯乙基)色酮（PEC）相关的生物合成途径在奇楠中的富集比普通种质更显著，且相关基因在受伤后在奇楠中的上调幅度明显更高。鉴定出16个可能参与沉香成分生物合成的候选基因，发现它们在受伤后在奇楠中的上调幅度高于普通种质。总体而言，这些结果有助于解释奇楠产香特性更高的原因，并有助于进一步了解沉香形成的机制。
