Yu Meng, Liu Yangyang, Feng Jian, Chen Deli, Yang Yun, Liu Peiwei, Yu Zhangxin, Wei Jianhe
Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education & National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.
Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine & Key Laboratory of State Administration of Traditional Chinese Medicine for Agarwood Sustainable Utilization, Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou 570311, China.
Int J Anal Chem. 2021 Apr 10;2021:5593730. doi: 10.1155/2021/5593730. eCollection 2021.
Wild Chi-Nan agarwood is regarded as the highest quality agarwood from spp. However, the comprehensive research on chemical composition of wild Chi-Nan agarwood is limited. An integrated strategy using SHS-GC-MS and UPLC-Q/Tof-MS was applied to explore the phytochemical characteristics of a kind of agarwood induced from a newly identified germplasm of Chi-Nan Progenesis QI and MS-Dial were used to preprocess the UPLC-Q/Tof-MS and GC-MS raw data, respectively. Principle component analysis (PCA) and orthogonal partial least squares to latent structure-discriminant analysis (OPLS-DA) models were built to discriminate Chi-Nan agarwood from ordinary agarwood and to screen potential distinguishing components between them. In this study, we clarified the distinguishing differences between Chi-Nan agarwood and ordinary agarwood. The difference is mainly manifested in the average contents of 2-(2-phenylethyl)chromone and 2-[2-(4'-methoxybenzene)ethyl]chromone, which are 170 and 420 times higher in Chi-Nan agarwood than in ordinary agarwood, respectively, while the contents of 5,6,7,8-diepoxy-2-(2-phenylethyl)chromones(DEPECs), 5,6-epoxy-2-(2-phenylethyl)chromones(EPECs), and 5,6,7,8-tetrahydro-2-(2-phenylethyl)chromones(THPECs) such as agarotetrol are extremely low. The content of the main sesquiterpenes in Chi-Nan agarwood was higher than that in ordinary agarwood, especially in regard to guaiane and eudesmane derivatives. In addition, there were significant differences in the contents of low-molecular-weight aromatic compounds such as 2-methyl-4H-1-benzopyran-4-one, 4-methoxybenzaldehyde, and 2-hydroxybenzaldehyde between Chi-Nan agarwood and ordinary agarwood. All the mentioned main chemical characteristics of this new Chi-Nan agarwood were coincident with those of the rare wild Chi-Nan agarwood from , , and . We reported differences in 2-(2-phenylethyl)chromones, sesquiterpenes, and low-molecular-weight aromatic compounds between Chi-Nan agarwood and ordinary agarwood from for the first time; it is necessary to evaluate the agarwood from the new-found Chi-Nan germplasm.
野生奇楠沉香被认为是瑞香科植物中品质最高的沉香。然而，对野生奇楠沉香化学成分的综合研究有限。采用SHS-GC-MS和UPLC-Q/Tof-MS相结合的策略，探究一种由新鉴定的奇楠种质诱导产生的沉香的植物化学特征。分别使用Progenesis QI和MS-Dial对UPLC-Q/Tof-MS和GC-MS原始数据进行预处理。建立主成分分析（PCA）和正交偏最小二乘法判别分析（OPLS-DA）模型，以区分奇楠沉香和普通沉香，并筛选它们之间潜在的区分成分。在本研究中，我们阐明了奇楠沉香和普通沉香之间的显著差异。差异主要体现在2-(2-苯乙基)色酮和2-[2-(4'-甲氧基苯)乙基]色酮的平均含量上，奇楠沉香中的这两种成分分别比普通沉香高170倍和420倍，而5,6,7,8-二环氧-2-(2-苯乙基)色酮（DEPECs）、5,6-环氧-2-(2-苯乙基)色酮（EPECs）和5,6,7,8-四氢-2-(2-苯乙基)色酮（THPECs）如agarotetrol的含量极低。奇楠沉香中主要倍半萜的含量高于普通沉香，尤其是愈创木烷型和桉叶烷型衍生物。此外，奇楠沉香和普通沉香之间低分子量芳香化合物如2-甲基-4H-1-苯并吡喃-4-酮、4-甲氧基苯甲醛和2-羟基苯甲醛的含量也存在显著差异。这种新奇楠沉香的所有上述主要化学特征与来自、和的珍稀野生奇楠沉香一致。我们首次报道了奇楠沉香和普通沉香在2-(2-苯乙基)色酮、倍半萜和低分子量芳香化合物方面的差异；有必要对新发现的奇楠种质的沉香进行评估。
