Nair M P, Schwartz S A, Menon M
Cell Immunol. 1985 Aug;94(1):159-71. doi: 10.1016/0008-8749(85)90093-0.
Cord blood lymphocytes (CBL) were compared with adult peripheral blood lymphocytes (a-PBL) for their: (i) natural killer (NK) and antibody-dependent cellular cytotoxic (ADCC) activities, (ii) target-binding capacity, (iii) ability to induce soluble natural killer cytotoxic factor (NKCF), (iv) interferon (IFN)-, interleukin 2 (IL-2)-, and lectin-induced augmentation of NK activity, and (v) ability to produce IFN against tumor targets in vitro. CBL depleted of adherent cells and Percoll-separated, NK-enriched subpopulations demonstrated significantly lower NK, ADCC, and target-binding activities compared to a-PBL. CBL produced significantly lower levels of NKCF directed against K562 tumor targets in comparison with a-PBL. Although the NK activity of CBL was not stimulated by either IFN or IL-2 to the same levels shown by a-PBL, the percentage enhancement of cytotoxicity of CBL by IFN and IL-2 was greater than that of a-PBL. Lectin-induced enhancement of cytotoxicity was significantly greater for CBL in comparison with a-PBL. Further, the ability of CBL lymphocytes to produce IFN-gamma in vitro against K562 target cells was significantly lower than that of adult PBL. These studies suggest an association between decreased NK, ADCC, and target-binding activities, induction of NKCF and IFN production by CBL, and increased susceptibility of neonates to infection.
对脐血淋巴细胞（CBL）和成人外周血淋巴细胞（a-PBL）进行了以下方面的比较：（i）自然杀伤（NK）和抗体依赖性细胞毒性（ADCC）活性；（ii）靶细胞结合能力；（iii）诱导可溶性自然杀伤细胞毒性因子（NKCF）的能力；（iv）干扰素（IFN）-、白细胞介素2（IL-2）以及凝集素诱导的NK活性增强；（v）体外针对肿瘤靶细胞产生IFN的能力。与a-PBL相比，去除贴壁细胞并经Percoll分离的富含NK的CBL亚群表现出显著更低的NK、ADCC和靶细胞结合活性。与a-PBL相比，CBL产生的针对K562肿瘤靶细胞的NKCF水平显著更低。尽管CBL的NK活性未被IFN或IL-2刺激至a-PBL所显示的相同水平，但IFN和IL-2对CBL细胞毒性的增强百分比大于a-PBL。与a-PBL相比，凝集素诱导的CBL细胞毒性增强更为显著。此外，CBL淋巴细胞体外针对K562靶细胞产生IFN-γ的能力显著低于成人PBL。这些研究表明，CBL的NK、ADCC和靶细胞结合活性降低、NKCF诱导以及IFN产生与新生儿对感染易感性增加之间存在关联。
