Montagna D, Maccario R, Ugazio A G, Mingrat G, Burgio G R
Department of Pediatrics, University of Pavia, Italy.
Clin Exp Immunol. 1988 Jan;71(1):177-81.
In 28 healthy full-term newborns the percentage of circulating cells expressing the Leu7 antigen, the marker of natural killer (NK) cells, was significantly lower than in healthy adults. However, newborns and adults did not differ with regard to the percentage of cells reacting with the Leulla, Leullc and TEC NK-1, monoclonal antibodies directed against the IgG Fc receptor of killer cells. Spontaneous NK activity of neonatal cells was profoundly reduced compared to the adult. In contrast, antibody dependent cellular cytotoxicity and NK-like activity generated in mixed lymphocyte cultures were similar in the two groups and lymphokine-activated killer cell (LAK) activity was high in the neonate. Natural killing is thought to play an important role in antiviral immunity since the neonate has a deficient capacity to deal with viral infections. Consequently, the present data indicate either that spontaneous NK is the most informative in vitro measure of newborn natural cytotoxicity in vivo, or, alternatively, that natural killing is not as important in antiviral immunity as previously suggested.
在28名健康足月新生儿中，表达自然杀伤（NK）细胞标志物Leu7抗原的循环细胞百分比显著低于健康成年人。然而，新生儿和成年人在与Leulla、Leullc和TEC NK-1反应的细胞百分比方面没有差异，这三种单克隆抗体针对杀伤细胞的IgG Fc受体。与成年人相比，新生儿细胞的自发NK活性显著降低。相比之下，两组中抗体依赖性细胞毒性和混合淋巴细胞培养中产生的NK样活性相似，且新生儿的淋巴因子激活杀伤细胞（LAK）活性较高。由于新生儿应对病毒感染的能力不足，自然杀伤被认为在抗病毒免疫中起重要作用。因此，目前的数据表明，要么自发NK是体内新生儿自然细胞毒性最具信息量的体外测量指标，要么自然杀伤在抗病毒免疫中的重要性不如先前认为的那样。
