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低毒病毒1（ThHV1）及其缺陷RNA ThHV1-S对T-51抗真菌活性和代谢组的影响

The Effect of Hypovirus 1 (ThHV1) and Its Defective RNA ThHV1-S on the Antifungal Activity and Metabolome of T-51.

作者信息

You Jiaqi, Hu Zheng, Li Chaohan, Yang Hongjuan, Zhu Lihua, Cao Biting, Song Ronghao, Gu Weihong

机构信息

Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai Key Lab of Protected Horticultural Technology, Shanghai 201106, China.

Institute for Agri-Food Standards and Testing Technology, Shanghai Academy of Agricultural Science, Shanghai 201106, China.

出版信息

J Fungi (Basel). 2023 Jan 28;9(2):175. doi: 10.3390/jof9020175.

DOI:10.3390/jof9020175
PMID:36836290
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9959424/
Abstract

Mycoviruses widely exist in filamentous fungi and sometimes cause phenotypic changes in hosts. hypovirus 1 (ThHV1) and its defective RNA ThHV1-S were found in and exhibited high transmissibility. In our previous study, ThHV1 and ThHV1-S were transferred to an excellent biological control agent T-51 to form a derivative strain 51-13. In this study, we assessed the metabolic changes in strain 51-13 and antifungal activity of its culture filtrate (CF) and volatile organic compounds (VOCs). The antifungal activity of CF and VOCs of T-51 and 51-13 was different. Compared with the CF of T-51, that of 51-13 exhibited high inhibitory activity against , , and but low inhibitory activity against and . The VOCs of 51-13 exhibited high inhibitory activity against but low inhibitory activity against . The transcriptomes of T-51 and 51-13 were compared; 5531 differentially expressed genes (DEGs) were identified in 51-13 with 2904 up- and 2627 downregulated genes. In KEGG enrichment analysis, 1127 DEGs related to metabolic pathways (57.53%) and 396 DEGs related to biosynthesis of secondary metabolites (20.21%) were clearly enriched. From the CF of T-51 and 51-13, 134 differential secondary metabolites (DSMs) were detected between T-51 and 51-13 with 39 up- and 95 downregulated metabolites. From these, 13 upregulated metabolites were selected to test their antifungal activity against . Among them, indole-3-lactic acid and p-coumaric acid methyl ester (MeCA) exhibited strong antifungal activity. The IC of MeCA was 657.35 μM and four genes possibly related to the synthesis of MeCA exhibited higher expression in 51-13 than in T-51. This study revealed the mechanism underlying the increase in antifungal activity of T-51 because of the mycovirus and provided novel insights in fungal engineering to obtain bioactive metabolites via mycoviruses.

摘要

真菌病毒广泛存在于丝状真菌中，有时会导致宿主表型发生变化。在[具体真菌名称未给出]中发现了低毒病毒1（ThHV1）及其缺陷RNA ThHV1-S，且其具有高传播性。在我们之前的研究中，ThHV1和ThHV1-S被转移到一种优良的生防菌T-51中，形成了衍生菌株51-13。在本研究中，我们评估了菌株51-13的代谢变化及其培养滤液（CF）和挥发性有机化合物（VOCs）的抗真菌活性。T-51和51-13的CF和VOCs的抗真菌活性有所不同。与T-51的CF相比，51-13的CF对[具体真菌名称未给出1]、[具体真菌名称未给出2]和[具体真菌名称未给出3]表现出高抑制活性，但对[具体真菌名称未给出4]和[具体真菌名称未给出5]表现出低抑制活性。51-13的VOCs对[具体真菌名称未给出6]表现出高抑制活性，但对[具体真菌名称未给出7]表现出低抑制活性。比较了T-51和51-13的转录组；在51-13中鉴定出5531个差异表达基因（DEGs），其中2904个基因上调，2627个基因下调。在KEGG富集分析中，1127个与代谢途径相关的DEGs（57.53%）和396个与次生代谢物生物合成相关的DEGs（20.21%）明显富集。从T-51和51-13的CF中，检测到T-51和51-13之间有134种差异次生代谢物（DSMs），其中39种代谢物上调，95种代谢物下调。从中选择13种上调的代谢物测试它们对[具体真菌名称未给出8]的抗真菌活性。其中，吲哚-3-乳酸和对香豆酸甲酯（MeCA）表现出较强的抗真菌活性。MeCA的IC为657.35μM，4个可能与MeCA合成相关的基因在51-13中的表达高于T-51。本研究揭示了由于真菌病毒导致T-51抗真菌活性增加的机制，并为通过真菌病毒获得生物活性代谢物的真菌工程提供了新的见解。

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