Department of Public Health and Clinical Medicine, Umeå University, Umeå, Sweden.
Department of Medical Biosciences, Umeå University, Umeå, Sweden.
Scand J Clin Lab Invest. 2024 Oct;84(6):405-409. doi: 10.1080/00365513.2024.2403005. Epub 2024 Sep 12.
Frozen saliva samples are often used for later determination of salivary glucocorticoids in research studies on stress and endocrine disorders. We studied the stability of cortisol and cortisone in saliva after six years of storage at -80 °C by repeated analysis of 153 stored aliquots, collected with Salivette, using liquid chromatography tandem mass spectrometry. We found a very high agreement between the first and the repeated measurement after six years at -80 °C, for both cortisol and cortisone concentrations (= 0.96 and = 0.98, respectively). Passing-Bablok regression equations were = 0.02 + 1.00 and = 0.02 + 1.14 for cortisol and cortisone, respectively. We conclude that salivary cortisol and cortisone concentrations remain essentially unaltered after six years of storage at -80 °C.
冷冻唾液样本常用于研究应激和内分泌紊乱相关的医学学术文献中的唾液皮质醇。我们通过重复分析使用 Salivette 收集的 153 份冷藏样本，使用液相色谱串联质谱法，研究了在 -80°C 下储存 6 年后，皮质醇和可的松在唾液中的稳定性。我们发现，在 -80°C 下储存 6 年后，皮质醇和可的松的浓度在第一次和重复测量之间非常一致（分别为 = 0.96 和 = 0.98）。皮质醇和可的松的通过 Babolok 回归方程分别为 = 0.02 + 1.00 和 = 0.02 + 1.14。我们得出结论，在 -80°C 下储存 6 年后，唾液皮质醇和可的松浓度基本保持不变。
