Wannassi Takwa, Sayadi Ahmed, Abbes Khaled, Djebbi Salma, Naccache Chahnez, Khemakhem Maha Mezghani, Chermiti Brahim
Department of Biological Sciences and Plant Protection, High Agronomic Institute of Chott-Mariem, University of Sousse, 4042, Sousse, Tunisia.
Laboratory of Biochemistry and Biotechnology (LR01ES05), Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis, Tunisia.
Int Microbiol. 2024 Sep 12. doi: 10.1007/s10123-024-00593-9.
Obligate endosymbiont bacteria associated with insects are naturally providing their hosts with essential nutrients such as vitamins and amino acids and biological services including protection from pathogens. In this study, we aimed to investigate the presence of Wolbachia infection among males and females of the parasitic apricot seed wasp (ASW) Eurytoma samsonowi Vassiliev (Vassiliev Petrograd 11: 1-15, 1915) (Hymenoptera: Eurytomidae), a very harmful pest of apricot (Prunus armeniaca), in the oasis of Gafsa, Southern-West of Tunisia. The detection of Wolbachia infection was assessed based on the amplification of the Wolbachia surface protein (wsp) gene and a multilocus sequence typing (MLST) as a universal genotyping tool for Wolbachia involving the analyses of genes gatB, coxA, hcpA, fbpA, and ftsz. Confirming the screening results, Wolbachia was detected in the natural apricot wasp for the first time, with a significant difference between males (5%) and females (59%) based on wsp gene. All Wolbachia strains identified in E. samsonowi were clustered among supergroups B of Wolbachia.
与昆虫相关的专性内共生细菌自然地为其宿主提供必需营养物质，如维生素和氨基酸，以及生物服务，包括抵御病原体。在本研究中，我们旨在调查寄生性杏核蜂（ASW）桑氏广肩小蜂（Eurytoma samsonowi Vassiliev）（瓦西里耶夫，彼得格勒11: 1 - 15，1915年）（膜翅目：广肩小蜂科）的雄性和雌性个体中沃尔巴克氏体感染的情况，桑氏广肩小蜂是突尼斯西南部加夫萨绿洲中杏树（Prunus armeniaca）的一种极具危害性的害虫。基于沃尔巴克氏体表面蛋白（wsp）基因的扩增以及多位点序列分型（MLST）（一种用于沃尔巴克氏体的通用基因分型工具，涉及对gatB、coxA、hcpA、fbpA和ftsz基因的分析）来评估沃尔巴克氏体感染的检测情况。通过wsp基因确认筛选结果，首次在自然状态下的杏核蜂中检测到沃尔巴克氏体，基于wsp基因，雄性（5%）和雌性（59%）之间存在显著差异。在桑氏广肩小蜂中鉴定出的所有沃尔巴克氏体菌株都聚集在沃尔巴克氏体的B超群中。
