Niboucha Nissa, Jubinville Éric, Péloquin Laurence, Clop Amandine, Labrie Steve, Goetz Coralie, Fliss Ismaïl, Jean Julie
Département Des Sciences Des Aliments, Université Laval, Québec, QC, G1V 0A6, Canada.
Institut Sur La Nutrition Et Les Aliments Fonctionnels (INAF), Québec, QC, G1V 0A6, Canada.
Probiotics Antimicrob Proteins. 2024 Sep 12. doi: 10.1007/s12602-024-10351-y.
Biofilms may contain pathogenic and spoilage bacteria and can become a recurring problem in the dairy sector, with a negative impact on product quality and consumer health. Peracetic acid (PAA) is one of the disinfectants most frequently used to control biofilm formation and persistence. Though effective, it cannot be used at high concentrations due to its corrosive effect on certain materials and because of toxicity concerns. The aim of this study was to test the possibility of PAA remaining bactericidal at lower concentrations by using it in conjunction with reuterin (3-hydroxypropionaldehyde). We evaluated the efficacy of PAA in pure form or as BioDestroy, a PAA-based commercial disinfectant, on three-species biofilms formed by dairy-derived bacteria, namely Pseudomonas azotoformans PFlA1, Serratia liquefaciens Sl-LJJ01, and Bacillus licheniformis Bl-LJJ01. Minimum inhibitory concentrations of the three agents were determined for each bacterial species and the fractional inhibitory concentrations were then calculated using the checkerboard assay. The minimal biofilm eradication concentration (MBEC) of each antibacterial combination was then calculated against mixed-species biofilm. PAA, BioDestroy, and reuterin showed antibiofilm activity against all bacteria within the mixed biofilm at respectively 760 ppm, 450 ppm, and 95.6 mM. The MBEC was lowered significantly to 456 ppm, 337.5 ppm, and 71.7 mM, when exposed to reuterin for 16 h followed by contact with disinfectant. Combining reuterin with chemical disinfection shows promise in controlling biofilm on food contact surfaces, especially for harsh or extended treatments. Furthermore, systems with reuterin encapsulation and nanotechnologies could be developed for sustainable antimicrobial efficacy without manufacturing disruptions.
生物膜可能含有致病和腐败细菌，会成为乳制品行业反复出现的问题，对产品质量和消费者健康产生负面影响。过氧乙酸（PAA）是最常用于控制生物膜形成和持续存在的消毒剂之一。尽管有效，但由于其对某些材料的腐蚀作用以及毒性问题，不能高浓度使用。本研究的目的是通过将PAA与罗伊氏菌素（3-羟基丙醛）联合使用，测试其在较低浓度下保持杀菌作用的可能性。我们评估了纯形式的PAA或作为BioDestroy（一种基于PAA的商业消毒剂）对由乳制品来源细菌形成的三物种生物膜的功效，这些细菌分别是产氮假单胞菌PFlA1、液化沙雷氏菌Sl-LJJ01和地衣芽孢杆菌Bl-LJJ01。测定了每种细菌对这三种药剂的最低抑菌浓度，然后使用棋盘法计算部分抑菌浓度。然后计算每种抗菌组合对混合物种生物膜的最低生物膜根除浓度（MBEC）。PAA、BioDestroy和罗伊氏菌素分别在760 ppm、450 ppm和95.6 mM时对混合生物膜中的所有细菌表现出抗生物膜活性。当暴露于罗伊氏菌素16小时后再接触消毒剂时，MBEC显著降低至456 ppm、337.5 ppm和71.7 mM。将罗伊氏菌素与化学消毒相结合在控制食品接触表面的生物膜方面显示出前景，特别是对于苛刻或延长的处理。此外，可以开发具有罗伊氏菌素封装和纳米技术的系统，以实现可持续的抗菌效果而不影响生产。
