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使用 Opal 多重免疫荧光技术表征小鼠肠上皮细胞谱系的方案。

Protocol to characterize mouse intestinal epithelial cell lineage using Opal multiplex immunofluorescence.

机构信息

Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USA.

Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USA.

出版信息

STAR Protoc. 2024 Sep 20;5(3):103303. doi: 10.1016/j.xpro.2024.103303. Epub 2024 Sep 12.

DOI:10.1016/j.xpro.2024.103303
PMID:39264806
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11422768/
Abstract

Applying Opal multiplex immunofluorescence (OMI) to characterize intestinal tissues of genetically engineered mouse models provides an excellent tool for studying complex processes. However, detecting appropriate signals from multiple target molecules is challenging. Here, we present a protocol to characterize mouse intestinal epithelial cell lineage using OMI. We describe steps for processing small intestine and colonic mouse tissues and designing and optimizing panels for OMI in mouse intestinal tissues. We then detail procedures for performing a quantitative evaluation of acquired images. For complete details on the use and execution of this protocol, please refer to Kinoshita et al..

摘要

应用 Opal 多重免疫荧光（OMI）技术来描绘遗传工程小鼠模型的肠道组织，为研究复杂的生物学过程提供了一个非常有用的工具。然而，从多个靶标分子中检测到合适的信号是具有挑战性的。在此，我们提出了一种利用 OMI 来描绘小鼠肠道上皮细胞谱系的方案。我们描述了从小鼠的小肠和结肠组织中处理样本以及为 OMI 设计和优化面板的步骤。然后，我们详细介绍了对获得的图像进行定量评估的程序。如需详细了解该方案的使用和执行，请参考 Kinoshita 等人的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaea/11422768/314cc0173c03/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaea/11422768/7ff1f0ab0929/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaea/11422768/79f943e1b615/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaea/11422768/ebbb1182ab0f/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaea/11422768/229ba827ddb1/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaea/11422768/cbe57ff5121e/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaea/11422768/f04701cd1a79/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaea/11422768/10775a908d93/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaea/11422768/314cc0173c03/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaea/11422768/7ff1f0ab0929/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaea/11422768/79f943e1b615/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaea/11422768/ebbb1182ab0f/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaea/11422768/229ba827ddb1/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaea/11422768/cbe57ff5121e/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaea/11422768/f04701cd1a79/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaea/11422768/10775a908d93/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaea/11422768/314cc0173c03/gr7.jpg

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本文引用的文献

1
Epithelial aPKC deficiency leads to stem cell loss preceding metaplasia in colorectal cancer initiation.上皮非典型蛋白激酶C缺乏导致结直肠癌起始过程中化生之前的干细胞丢失。
Dev Cell. 2024 Aug 5;59(15):1972-1987.e8. doi: 10.1016/j.devcel.2024.05.001. Epub 2024 May 29.
2
Hyaluronan driven by epithelial aPKC deficiency remodels the microenvironment and creates a vulnerability in mesenchymal colorectal cancer.上皮细胞 aPKC 缺乏导致透明质酸重塑微环境，并在结直肠间质肿瘤中产生脆弱性。
Cancer Cell. 2023 Feb 13;41(2):252-271.e9. doi: 10.1016/j.ccell.2022.11.016. Epub 2022 Dec 15.
3
Simultaneous Loss of Both Atypical Protein Kinase C Genes in the Intestinal Epithelium Drives Serrated Intestinal Cancer by Impairing Immunosurveillance.
肠上皮中非典型蛋白激酶 C 基因的同时缺失通过损害免疫监视导致锯齿状肠道肿瘤。
Immunity. 2018 Dec 18;49(6):1132-1147.e7. doi: 10.1016/j.immuni.2018.09.013. Epub 2018 Dec 11.