Li Shuqin, You Siyuan, Mao Kairui
State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, Xiamen University, Xiamen, Fujian 361102, China.
State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, Xiamen University, Xiamen, Fujian 361102, China; Department of Gastroenterology, The National Key Clinical Specialty, Zhongshan Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, Fujian 361004, China; Xiang'an Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, Fujian 361102, China.
STAR Protoc. 2025 Jun 20;6(2):103776. doi: 10.1016/j.xpro.2025.103776. Epub 2025 Apr 16.
The tumor microenvironment (TME) plays a critical role in both tumor progression and therapeutic efficacy. Here, we present a protocol to perform transcription factor and surface marker staining for a comprehensive analysis of the TME of mouse intestinal tumors by combining multiplex immunofluorescence imaging with the "Swiss roll" technique. We describe steps for sample preparation, sectioning, staining, and mounting. We then detail procedures for imaging and data processing. This protocol supports quantitative assessments such as calculating cell-to-cell distances. For complete details on the use and execution of this protocol, please refer to You et al..
肿瘤微环境（TME）在肿瘤进展和治疗效果中都起着关键作用。在此，我们提出一种方案，通过将多重免疫荧光成像与“瑞士卷”技术相结合，对小鼠肠道肿瘤的TME进行全面分析，以进行转录因子和表面标志物染色。我们描述了样品制备、切片、染色和封片的步骤。然后详细介绍成像和数据处理程序。该方案支持诸如计算细胞间距离等定量评估。有关本方案使用和执行的完整详细信息，请参考You等人的研究。
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