Campbell H D, Ymer S, Fung M C, Young I G
Eur J Biochem. 1985 Jul 15;150(2):297-304. doi: 10.1111/j.1432-1033.1985.tb09020.x.
Southern hybridization analysis using a probe derived from a murine interleukin-3 (IL-3) cDNA clone revealed the presence of a single IL-3 gene in the haploid murine genome. An 8600-base-pair (8.6-kb) murine genomic EcoRI fragment containing the IL-3 gene was isolated by screening a library of size-fractionated genomic EcoRI fragments cloned in lambda gtWES X lambda B. The nucleotide sequence of a 3.5-kb region of the cloned DNA encompassing the IL-3 gene was determined. The gene contains four introns of 96, 993, 135 and 122 base pairs (bp), located within the coding region. The large intron contains 12 copies of a 14-15-bp tandem repeating sequence which resembles a human cellular homologue of a BKV enhancer sequence. The nucleotide sequence of the exons agrees exactly with that of an IL-3 cDNA cloned from WEHI-3, a tumorigenic cell line which over-produces IL-3, establishing that the unprocessed primary structure of IL-3 is identical in WEHI-3 and in BALB/c mice. Southern hybridization has revealed genomic alteration in the vicinity of the IL-3 gene in WEHI-3 cells.
使用源自小鼠白细胞介素-3（IL-3）cDNA克隆的探针进行的Southern杂交分析表明，在单倍体小鼠基因组中存在单个IL-3基因。通过筛选克隆于λgtWES×λB中的大小分级基因组EcoRI片段文库，分离出一个包含IL-3基因的8600碱基对（8.6 kb）小鼠基因组EcoRI片段。测定了包含IL-3基因的克隆DNA 3.5 kb区域的核苷酸序列。该基因在编码区内含有四个分别为96、993、135和122碱基对（bp）的内含子。大内含子包含12个14 - 15 bp的串联重复序列拷贝，其类似于BKV增强子序列的人类细胞同源物。外显子的核苷酸序列与从过量产生IL-3的致瘤细胞系WEHI-3克隆的IL-3 cDNA的序列完全一致，这表明在WEHI-3和BALB/c小鼠中，IL-3未加工的一级结构是相同的。Southern杂交显示WEHI-3细胞中IL-3基因附近存在基因组改变。
