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大鼠白细胞介素-3基因的克隆与表达

Cloning and expression of the rat interleukin-3 gene.

作者信息

Cohen D R, Hapel A J, Young I G

出版信息

Nucleic Acids Res. 1986 May 12;14(9):3641-58. doi: 10.1093/nar/14.9.3641.

DOI:10.1093/nar/14.9.3641
PMID:3086845
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC339805/
Abstract

Genomic clones carrying the rat interleukin-3 (IL-3) gene have been isolated and the nucleotide sequence of the gene determined. Alignment of this sequence with that of the mouse IL-3 gene has allowed the structure of the rat IL-3 gene to be deduced. The intron-exon boundaries are conserved and extensive nucleotide homology (approx 90%) is present in the 5' flanking region and the portion of the gene coding for the signal peptide. Several proposed regulatory sequences are conserved and an analogous element to the tandem repeat in intron 2 of the mouse gene is also present. The predicted amino acid sequence for mature rat IL-3 shows surprisingly low homology (54%) with its murine counterpart, although all four cysteine residues are conserved. The rat IL-3 gene was expressed in monkey COS-1 cells and colony assays established that rat IL-3 is a multi-lineage haemopoietic growth regulator. There was little cross-reactivity of the respective IL-3 species on mouse and rat bone marrow cells suggesting that rat IL-3, in concert with its receptor, has evolved significantly away from the mouse IL-3/receptor system.

摘要

携带大鼠白细胞介素-3（IL-3）基因的基因组克隆已被分离出来，并测定了该基因的核苷酸序列。将此序列与小鼠IL-3基因的序列进行比对，从而推断出大鼠IL-3基因的结构。内含子-外显子边界是保守的，并且在5'侧翼区域和编码信号肽的基因部分存在广泛的核苷酸同源性（约90%）。几个提议的调控序列是保守的，并且在小鼠基因内含子2中的串联重复类似元件也存在。成熟大鼠IL-3的预测氨基酸序列与其小鼠对应物的同源性惊人地低（54%），尽管所有四个半胱氨酸残基都是保守的。大鼠IL-3基因在猴COS-1细胞中表达，集落测定表明大鼠IL-3是一种多谱系造血生长调节因子。各自的IL-3物种在小鼠和大鼠骨髓细胞上几乎没有交叉反应性，这表明大鼠IL-3与其受体一起，已经从小鼠IL-3/受体系统中显著进化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9eed/339805/a71e0024408e/nar00278-0050-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9eed/339805/a71e0024408e/nar00278-0050-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9eed/339805/a71e0024408e/nar00278-0050-a.jpg

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